木犀草素改善低温下离体大鼠心脏保存效果的研究

Myocardial protective effects of luteolin on isolated rat heart in hypothermic preservation

目的:研究木犀草素是否能改善心脏停搏保存液(UW液)对离体大鼠心脏的低温保存效果。方法:将40只成年SD大鼠随机分成4组(n=10):对照组(UW组)、7.5μmol/L木犀草素小剂量组,15μmol/L木犀草素中剂量组及30μmol/L木犀草素大剂量组。利用Langendorff离体心脏灌流法,观察心脏在4℃含或不含木犀草素的UW液中保存12 h复灌60 min后心脏功能及超微结构变化,比较心脏冠脉流量(CF)、心肌含水量及冠脉流出液中磷酸肌酸激酶(CK)的释放量。结果:与对照组比较,添加木犀草素后,复灌期心脏的收缩功能(LVPSP,+dp/dtmax)与心脏舒张功能(-dp/dtmax)、冠脉流量在多个复灌时间点均优于对照组,心率在复灌60 min时也显著优于对照组;复灌过程中磷酸肌酸激酶的漏出量及低温保存后心脏超微结构的损伤也均明显低于对照组;随灌注时间延长木犀草素组心脏结构和功能的改善有剂量依赖性趋势;木犀草素对心肌含水量没有影响。结论:木犀草素能显著改善UW液对离体大鼠心脏的低温保存效果,对心脏有明显的保护作用,以30μmol/L的木犀草素大剂量组作用最显著。

Objective： To investigate the protective effect of luteolin on isolated rat heart in hypothermic preservation.Methods： Forty male SD rats were randomly divided into 4 groups（n=10）： control group,luteolin low-dose group（7.5 μmol/L）,middle-dose group（15 μmol/L） and high dose group（30 μmol/L）.Langendorff model of isolated rat heart was used.After 30 min basal perfusion,the hearts were stored in University of Wisconsin solution（UW solution） at 4°C with luteolin（7.5,15 and 30 μmol/L） or without luteolin for 12 h and followed by 60 min reperfusion.The recovery of cardiac contractile and diastolic function,coronary flow（CF）,creatine kinase（CK） leakage in the coronary effluent,myocardial water content were determined.The myocardial ultrastructure was also observed.Results： The results revealed that luteolin improved the recovery of left ventricular peak systolic pressure and ±dp/dtmax dose-dependently and increased coronary flow.The leakage of creatine kinase in the coronary effluent was significantly reduced in luteolin-added hearts.Impairment of myocardial ultrastructure after 12 h hypothermic preservation was obviously alleviated in hearts luteolin-added group compared with that in control group.There were no differences between the groups in myocardial water contents.Conclusion： Luteolin as a supplementation in cardiac preservation solution can significantly improve the hypothermic preservation effects on rat heart and have myocardial protection effect,especially in luteolin-added with 30 μmol/L.