海参多糖对急性不完全性脑缺血的保护及抗凝作用的研究

Protective effects and anticoagulation effect of polysaccharides from the sea cucumber on acute incomplete cerebral ischemia in rats

目的:研究海参多糖(PSU)对大鼠急性不完全性脑缺血(AICI)损伤的保护作用及抗凝血作用。方法:SD大鼠60只,随机分为5组(n=12):假手术组、模型组、海参多糖低(30 mg/(kg.d))、中(60mg/(kg.d))、高(120mg/(kg.d))剂量组。双侧颈总动脉永久性结扎法复制大鼠急性不完全性脑缺血模型,观察海参多糖对大鼠行为障碍、脑组织含水量、血清C-反应蛋白(CRP)水平的变化情况,以及对凝血酶原时间(PT)、活化的部分凝血酶时间(APTT)、凝血酶时间(TT)和纤维蛋白原含量(FIB)等血液凝血指标的影响。结果:海参多糖可改善行为学障碍,减少脑组织含水量,降低血清CRP水平;可显著延长APTT,TT时间,减少FIB含量(P<0.05)。结论:海参多糖能明显减轻大鼠急性不完全性脑缺血损伤,并具有显著的抗凝血作用。

Objective： To investigate the protective effect and anticoagulation effect of polysaccharides from the sea cucumber（PSU） on acute incomplete cerebral ischemia（AICI）.Methods： Adult SD rats were randomly divided into 5 group（n=12）：sham operation group,model group,low-dose（30 mg/（kg·d））,middle-dose（60 mg/（kg·d）） and high-dose（120 mg/（kg·d）） groups.The cerebral ischemia rat model was established by permanently ligating the common carotid arteries on both sides of rats.We observed the change of behavior disturbance,brain water content,the levels of serum C-reactive protein（CRP） as well as the effects on prothrombin time（PT）,activated partial thromboplastin time（APTT）,thrombin time（TT）,fibrinogen（FIB）.Results： Due to the use of polysaccharides,the behavioral disturbance was improved,brain water content and the levels of serum CRP were significantly decreased.Also PSU significantly prolonged APTT,TT and reduced the content of FIB（P0.05）.Conclusion： PSU has protective effect on acute incomplete cerebral ischemia injured rats and significant anticoagulation effect.