摘要
为了检验大肠杆菌O157∶H7胶体金免疫层析检测试纸条在感染动物排菌中的应用效果,将牛和小鼠采用灌胃攻毒方式试验感染大肠杆菌O157∶H7,用大肠杆菌O157∶H7胶体金免疫层析检测试纸条、细菌鉴别培养基培养计数以及PCR 3种方法检测感染动物粪便中O157∶H7的排菌量和持续时间。牛在感染大肠杆菌O157∶H7后的第2天开始从粪便排菌,第4天达到峰值,排菌时间可持续28d;小鼠在感染O157∶H7后的第4小时开始从粪便排菌,第6小时达到峰值,排菌时间可持续15d。大肠杆菌O157∶H7胶体金免疫层析检测试纸条、细菌鉴别培养基培养计数以及PCR 3种方法的检测结果一致,但试纸条更简便、快捷、直观,1～5min可得出检测结果,便于现场检测样品中O157∶H7的快速筛查。
To investigate the application of colloidal gold immunochromatographic strip for the detection of animals inoculated with Escherichia coli O157∶H7,cattle and mice were inoculated intragastrically with E.coli O157∶H7,and their faeces were detected using three methods of colloidal gold immunochro-matographic strip,counting bacterial colony in differential medium and polymerase chain reaction（PCR）.E.coli O157∶H7 in the faeces of the inoculated cattle could be detected on 2 d after inoculation with the peak at 4 d after inoculation and then it dropped down gradually till to 28 d.In the inoculated mice,O157∶H7 could be detected in faeces at 4 h and the peak levels at 6 h after inoculation,which dropped down gra-dually till to 15 d.Although the results of the three methods were consistent with each other,the colloidal gold immunochromatographic strip was more convenient and rapid for the clinical diagnosis and field investigation of E.coli O157∶H7 infection or contamination than other two methods.The detection result could be obtained within 1 to 5 minutes.
出处
《中国兽医科学》
CAS
CSCD
北大核心
2012年第3期285-289,共5页
Chinese Veterinary Science
基金
"十一五"国家科技支撑计划项目(2007BAD40B01)
