摘要
目的:研究葡萄糖对血浆中载脂蛋白的修饰作用,寻找标志性肽段,作为临床诊断糖尿病以及衰老相关疾病的生物标志物。方法:将蛋白质组学和生物信息学相结合,采用LC/MS联用技术寻找差异肽段。结果:载脂蛋白(APOA1)与葡萄糖发生糖基化反应,导致正常肽段的含量发生变化,并生成早期糖基化产物果糖胺。而糖尿病人体内的APOA1也同样会发生这种非酶糖基化修饰,其中的一些肽段也出现类似的变化情况。结论:筛选出五个肽段,将有可能作为临床诊断糖尿病以及与衰老相关疾病的生物标志物。
Objective：A group of typical peptides as biomarkers for clinical diagnose of diabetes and aging were investigated by studying glucose＇s modification on Apolipoprotein A-I（APOA1） of plasma.Method：Combination proteomics with bioinformatics,different peptides were screened by LC/MS.Result：Analysis of the LC/MS data showed that APOA1 be modified by glucose and fructosamine produce.The same modification also happened and some related peptides were found in diabetic patients.Conclusion：It is important for identifying the five peptides as biomarkers for clinical diagnose of diabetes and aging.
出处
《生物技术》
CAS
CSCD
北大核心
2012年第1期38-42,共5页
Biotechnology
基金
国家自然科学基金项目(No.20435020)资助
