亚微米造影剂在高强度聚焦超声消融兔VX2乳腺肿瘤中的应用研究

High-intensity focused ultrasound with submierobubbles to damage rabbit breast VX2 tumors

目的在高强度聚焦超声（HIFU）消融兔VX2乳腺肿瘤的过程中加入亚微米造影剂，探讨其对辐照效果的影响。方法制备亚微米造影剂，检测微泡性质。VX2乳腺肿瘤兔随机分组，在不同辐照剂量（150W、120w）和不同辐照时间（5s、3s）条件下，记录HIFU辐照前后灰阶变化，辐照后行病理检查。结果成功制备亚微米造影剂。HIFU＋亚微米造影剂组较HIFU＋PBS组能够有效提高HIFU的损伤面积，减少辐照强度，缩短辐照时间。结论亚微米造影剂不仅在诊断方面有特殊的应用价值，在超声治疗领域也有很好的应用前景。

Objective To observed the effect of high-intensity focused ultrasound （HIFU） in combination of submicrobubble on ablation of breast tumor VX2 in rabbits. Methods Submicrobubble was prepared,and the appearance, distribution, diameter, and zeta potential were measured. The rabbits were injected with the tumor tissue suspension in bilateral breast tissues. HIFU （150 W, 120 W, 5 s, 3 s） combined with submicrobubble to damage rabbit breast VX2 tumors. The coagulation necrosis was assessed by HE staining. The grayscale changes of the target area before and after HIFU irradiation were recorded automatically by computer. Results The diameter of the submicrobubble was 498.9 nm on average. HE staining showed the area of coagulation necrosis was significantly larger in the HIFU ＋ submicrobubble group than the HIFU ＋ PBS group. The injury range of 150 W irradiation was larger than that of 120 W irradiation,given the same irradiation time. The injury range of 5 s irradiation was larger than that of 3 s irradiation,given the same irradiation strength. The gray scale change in the target area was significantly higher in the HIFU ＋ submicrobubble group than the HIFU ＋ PBS group. Conclusions The submicrobubble exhibited sound imaging performance, and it can be use in the ultrasound therapeutics in the future.