呼吸道合胞病毒感染对哮喘小鼠RANTES表达的影响

Effects of respiratory syncytial virus infection on RANTES expression in murine model of asthma

目的研究呼吸道合胞病毒(RSV)感染对哮喘小鼠RANTES(regulated upon activation normal T cellexpressed and secreted)生成的影响。方法将BALB/c小鼠分为对照组、哮喘组、RSV感染组和RSV感染哮喘组4组,每组各8只。先采用卵清白蛋白致敏法制作小鼠哮喘模型,随后以RSV感染小鼠,RSV感染后24 h处死小鼠。检测小鼠支气管肺泡灌洗液(BALF)中RANTES及IFN-γ的浓度,并计数炎性细胞数。结果 RSV感染组和对照组,RSV感染哮喘组和哮喘组小鼠之间,BALF中的RANTES浓度差异均有统计学意义(P均<0.05);而对照组和哮喘组,RSV感染组和RSV感染哮喘组小鼠之间的RANTES浓度差异则无统计学意义(P均>0.05)。各组小鼠BALF中的IFN-γ浓度差异无统计学意义。哮喘组、RSV感染组、RSV感染哮喘组小鼠BALF中的各种炎性细胞计数均较对照组升高,差异均有统计学意义(P<0.05),哮喘小鼠以嗜酸性粒细胞增高为主,而RSV感染小鼠以中性粒细胞为主。结论感染RSV可使小鼠RANTES的表达增加,但哮喘未能使小鼠RANTES表达增加,提示RANTES增加可能与RSV感染有关,而与哮喘状态无关。

Objective To investigate the difference of RANTES（regulated on activation normal T-cell expressed and secreted）expression between asthmatic mice and control mice after respiratory syncytial virus（RSV）infection in murine model of asthma.Methods A murine model of asthma was made by sensitizing BALB/c mice with ovalbumin（OVA）.After infecting the asthmatic mice with RSV,the concentrations of RANTES and interferon-gamma in bronchoalveolar lavage fluid（BALF）were measured.In addition,the number of inflammatory cells in BALF after the viral infection was examined.Results Although both asthmatic mice and control mice showed increased RANTES expression after RSV infection,the difference between the two groups was not significant.When asthmatic mice were compared before and after RSV infection,the number of eosinophil was not changed but the number of neutrophil was increased.The interferon-gamma level was not raised by RSV infection in both asthmatic and control groups.Conclusions This study found that RSV infection does not lead to enhanced RANTES expression in asthmatic mice as compared to control mice.This finding implies that RSV infection induces RANTES expression regardless of asthma.