摘要
目的观察氟伐他汀预处理对大鼠脑缺血再灌注损伤后脑组织肿瘤坏死因子(TNF-α)及白介素-10(IL-10)含量变化,探讨氟伐他汀的脑保护机制。方法实验大鼠随机分为4组:假手术组(Sham组)、脑缺血再灌注组(I/R组)、溶剂对照组(Vehicle组)和氟伐他汀预处理组(Flu组)。线栓法制作大脑中动脉脑缺血再灌注损伤模型,Flu组在模型制备前用氟伐他汀连续灌胃14 d。于缺血2 h再灌注24 h后断头取脑,采用ELISA法、免疫组织化学法和半定量RT-PCR法,检测大鼠脑组织中TNF-α及IL-10表达的变化。结果与Sham组比较,I/R组和Vehicle组脑组织中TNF-α和IL-10的表达明显上调(P<0.05)。与I/R组和Vehicle组比较,Flu组脑组织中TNF-α的表达明显下调,IL-10的表达显著上调(P<0.05)。结论氟伐他汀对脑缺血再灌注损伤的保护作用,其作用机制可能与上调IL-10,下调TNF-α的表达有关。
To explore the protecting mechanism of fluvastatin on brain,we investigated the effects of fluvastatin preconditioning on the expression of tumor necrosis factor-α（TNF-α） and interleukin-10（IL-10） in rat brain with focal cerebral ischemia-reperfusion.Male SD adult rats were randomly divided into four groups： sham operation group（sham group）,focal cerebral ischemia-reperfusion group（I/R group）,vehicle administration group（vehicle group）,and fluvastatin preconditioning group（flu group）.The middle cerebral artery occlusion reperfusion model was set up by the suture method.The rats in the flu group were lavaged with fluvastatin for 14 consecutive days before the model setting-up.The expression levels of TNF-α and IL-10 in rat＇s brain were measured by ELISA,immunohistochemistry and RT-PCR.Compared with sham group,the expressions of TNF-α and IL-10 significantly up-regulated in I/R group and vehicle group（P 0.05）.Compared with I/R group and vehicle group,the expression of TNF-α expression significantly down-regulated while IL-10 expression significantly up-regulated in flu group（P 0.05）.From the result,we draw a conclusion that fluvastatin can lighten alleviate the neurons injury after focal cerebral ischemia-reperfusion injury in rats,partly by regulating the expression of IL-10 and TNF-α in the brain.
出处
《免疫学杂志》
CAS
CSCD
北大核心
2012年第4期309-311,316,共4页
Immunological Journal
