实时荧光定量PCR在侵袭性曲霉病诊断中的研究进展

Update of real-time PCR in the diagnosis of invasive Aspergillosis

实时荧光定量PCR自20世纪90年代初起被用于侵袭性曲霉病的诊断研究,具有较好的灵敏度和特异度,但由于技术操作多种多样,尚未统一,该技术仍未正式列入诊断标准。近年来,随着研究增加,对该技术操作的标准化研究取得了明显的进展。现从技术问题,包括标本种类、DNA提取方式、靶序列的选择、国际标准化研究进程以及该技术在侵袭性曲霉病的诊断价值和应用两个层面进行综述。

Quantitative real-time PCR（qPCR） has been applied in the research for early diagnosis of invasive aspergillosis（IA） for more than ten years with preferable sensitivities and specificities.However,various results were reported due to the variable procedures and specimens chosen.Standardization is needed for aspergillus identification.Progress and the utilization of qPCR in the diagnosis of IA were reviewed in this report.