紧密连接蛋白claudin-1与乳腺肿瘤的相关性研究

Study on Correlation Between Tight Junction Protein Claudin-1 and Breast Neoplasms

目的探讨紧密连接蛋白claudin-1在乳腺肿瘤组织中的表达及其与乳腺癌发生、发展的关系。方法应用组织芯片技术和免疫组织化学法研究89例乳腺癌和37例乳腺良性病变中claudin-1的表达情况,并统计分析其与乳腺癌淋巴结转移、TNM分期和肿块最大径以及组织学分级间的关系。结果 claudin-1在乳腺癌组织中的表达强度明显弱于乳腺良性病变者(χ2=19.20,P=0.000 2)。在有淋巴结转移的乳腺癌组织中claudin-1表达强度明显弱于无淋巴结转移者(χ2=3.85,P=0.049 7);TNM分期为Ⅲ期的乳腺癌组织中claudin-1表达强度分别弱于Ⅰ期(χ2=5.29,P=0.021 4)和Ⅱ期(χ2=7.46,P=0.006 3)。claudin-1表达强度在肿块最大径各组间(χ2=1.58,P=0.453 8)及组织学各分级之间(χ2=1.02,P=0.600 5)比较差异均无统计学意义。结论乳腺癌的发生、发展及转移可能与claudin-1的表达强度有关,可作为判断乳腺癌淋巴结转移和估计预后的参考指标之一。

Objective To investigate the expression of claudin-1 in breast tumor tissues and the relationship of development and progress of breast neoplasm.Methods The expressions of claudin-1 in 89 cases of breast cancer and 37 benign breast diseases were tested by tissue chip technology and immunohistochemistry.The relationships of claudin-1 expression to the lymph node metastasis,TNM staging,maximum diameter of the tumor,and histology grade were statistically analyzed.Results The expression of claudin-1 in the breast cancer was significantly lower than that in the benign breast disease（χ2=19.20,P=0.000 2）.The claudin-1 expression in the patients with lymph node metastasis was significantly lower than that without lymph node metastasis（χ2=3.85,P=0.049 7）.The claudin-1 expression in the stageⅢ of TNM staging was weaker than that in the stage Ⅰ（χ2=5.29,P=0.021 4） and stage Ⅱ（χ2=7.46,P=0.006 3）, respectively.There was no significant difference of the claudin-1 expression in the different maximum diameters of tumor（χ2=1.58,P=0.453 8） or histology grades（χ2=1.02,P=0.600 5）,respectively.Conclusions The expression of claudin-1 might be correlated with the occurrence,development,and metastasis in breast tumor.It may be one of the potential indicator for lymph node metastasis and prognosis assessment in breast cancer.