摘要
目的:探讨二烯丙基二硫(Diallyl disulfide)诱导白血病K562细胞凋亡的作用及其机制。方法:采用吖啶橙/溴化乙啶(AO/EB)染色法观察细胞凋亡形态学变化;DNA琼脂糖凝胶电泳测定DNA梯度带;RT-PCR法检测BAG-1、BAX基因的mRNA表达变化。结果:DADS可诱导K562细胞凋亡。其对K562细胞的凋亡效用与药物浓度、有明显依赖关系;DNA琼脂糖凝胶电泳示:40mg/LDADS作用K562细胞48小时后能够产生明显的梯形电泳图谱(DNA ladder):DADS作用48h后,BAX mRNA表达水平较对照组上调;BAG-1 mRNA较对照组下调(差异具有统计学意义,P<0.05)。结论:DADS能够诱导K562细胞凋亡,其凋亡机制可能与上调BAX,下调BAG-1有关。
Objective： To study the effects of diallyl dsulfide（DADS） on apoptosis of human leukemia K562 cells and possible mechanisms.Methods： The morphologic changes of leukemia K562 after DADS treatment were observed by AO/EB.DNA ladder was showed by agarose gelelectrophoresis.Bax,Bag-1 mRNA expression was estimated by reverse transcriptionpolymerase chain reaction（RT-PCR） 48 hours after DADS treatment.Results： The characteristics of apoptosis in k562 cells induced by DADS were observed.The leukemia K562 was treated with 40mg/L DADS for 48 hours reveal an obvious DNA ladder.Expression of Bax mRNA increased,while Bag-1 mRNA expression decreased significantly after DADS treatment for 48 hours compared with the control group（P0.05）.Conclusion： DADS can induce apoptosis of human leukemia K562 cells in a concent ration-dependent manner,possilbly through increasing Bax ex-pression and decreasing Bag-1 expression.
出处
《现代生物医学进展》
CAS
2012年第2期231-234,共4页
Progress in Modern Biomedicine
基金
湖南省高校科研经费项目(09C840)
