Hsp70对低氧大鼠海马DG区神经细胞p53、p-p38表达的研究

The Overexpression of HSP70 of the Neurocyte in the Area of Dentate Gyrus(DG)on the p53and p-p38 of Rat's Hippocampus Under Hypoxia

目的:探讨热休克蛋白70(HSP70)高表达对低氧大鼠海马DG区神经细胞p53、p-p38表达的影响。方法:将大鼠海马DG区神经细胞在41℃温浴1h诱导HSP70高表达,然后低氧培养,Western-blot检测细胞HSP70、p53、p-p38的表达。结果:温浴可以诱导HSP70高表达;HSP70高表达可抑制p53和p-p38表达。结论:HSP70保护低氧诱导的细胞凋亡可能与抑制p53和p-p38表达有关。

Objective： To investigate the effect of the HSP70 over-expression on the p53and p-p38 of the neurocyte in the area of dentate gyrus（DG）of rat＇s hippocampus under hypoxia.Methods： Neurocyte in the area of dentate gyrus（DG）of rat＇s hippocampus was treated in 41℃ for 1h to induced the over-expression of HSP70.Culturing in hypoxiathe.The level of p53 and p-p38 was detected by Western-blot.Results： Warm bath could induced the over-expression of HSP70;The overexpression of HSP70 down-regulated p53 and p-p38.Conclusions： The over-expression of HSP70 inhibited cell apoptosis from hypoxia maybe related to the downregulating of p53 and of p-p38.