摘要
目的:探讨热休克蛋白70(HSP70)高表达对低氧大鼠海马DG区神经细胞p53、p-p38表达的影响。方法:将大鼠海马DG区神经细胞在41℃温浴1h诱导HSP70高表达,然后低氧培养,Western-blot检测细胞HSP70、p53、p-p38的表达。结果:温浴可以诱导HSP70高表达;HSP70高表达可抑制p53和p-p38表达。结论:HSP70保护低氧诱导的细胞凋亡可能与抑制p53和p-p38表达有关。
Objective: To investigate the effect of the HSP70 over-expression on the p53and p-p38 of the neurocyte in the area of dentate gyrus(DG)of rat's hippocampus under hypoxia.Methods: Neurocyte in the area of dentate gyrus(DG)of rat's hippocampus was treated in 41℃ for 1h to induced the over-expression of HSP70.Culturing in hypoxiathe.The level of p53 and p-p38 was detected by Western-blot.Results: Warm bath could induced the over-expression of HSP70;The overexpression of HSP70 down-regulated p53 and p-p38.Conclusions: The over-expression of HSP70 inhibited cell apoptosis from hypoxia maybe related to the downregulating of p53 and of p-p38.
出处
《现代生物医学进展》
CAS
2012年第3期419-421,共3页
Progress in Modern Biomedicine
基金
全军重点基金课题(G-867)