DADS上调K562细胞p21^(WAF1)表达对细胞生长阻抑和凋亡的实验研究

Effect of Diallyl Disulfide on the Proliferation,Cell Cycle,Apoptosis of Human Leukemia Cell Line K562

目的:探讨二烯丙基二硫(DADS)对体外培养的人白血病细胞系K562细胞生长阻抑和凋亡作用及机制。方法:采用MTT分析法检测细胞活性、流式细胞术分析细胞周期及凋亡率、免疫组化检测p21WAF1基因表达。结果:1).DADS在10mg/L～80 mg/L范围内,对K562细胞的抑制作用呈剂量-时间依赖效应;2).不同浓度DADS作用于K562细胞24h后,细胞周期发生了变化:DADS可以将K562细胞阻滞于G2/M期;3).DADS浓度在10mg/L～80mg/L时作用K562细胞24h后,凋亡率逐渐升高,有显著的统计学意义(P<0.05或P<0.01);4).用浓度分别为0mg/L,20mg/L,40mg/L,80mg/L处理K562细胞24h后,p21WAF1蛋白表达上调,有统计学意义(P<0.05或P<0.01),溶媒组和阴性对照组无差别(P>0.05)。结论:DADS有抑制K562细胞增殖和促进K562细胞凋亡的作用。其作用的可能机制与上调细胞周期蛋白依赖性激酶抑制剂p21WAF1表达,从而诱使k562细胞阻滞于G2/M期有关。

Objective： To explore the mechanism of cell proliferation-inhabit and apotosis of human leukemia cell line K562 treated with diallyl disulfide（DADS）.Methods： MTT assay,flow cytometry and immunohistochemistry were employed to examine DADS-induced apoptosis and growth inhibition in human Leukemia cell Line K562.Rusults：1.DADS inhibited proliferation of K562 cells from 10～80mg.L-DADS in a dose-and time-dependent manner;2.K562 cells treated with DADS after the treatment for 24 hours significantly increased in the percentage of cells in the G2/M phase;3.From 10～80mg.L-DADS induced apotosis of K562 cells after the treatment for 24 hours,the difference is significant;4.DADS could up-regulate the level of protein of p21WAF1 in K562 cells.Conclusions：DADS has apparent effects of proliferation-inhabit and apoptosis on K562 cells in vitro,its anti-leukemia mechanisms may be related to up-regulate the gene of of p21WAF1 in K562 cells.