塞来西布及5-氟尿嘧啶对人胆管癌QBC939细胞生长抑制及诱导凋亡的影响

Effect of Celecoxib and 5-FU on Proliferation and Apoptosis of the Human Cholangiocarcinoma QBC-939 Cell Line

目的:研究塞来西布(Celecoxib)和5-氟尿嘧啶(5-FU)对人胆管癌QBC939细胞生长抑制和凋亡的影响。方法:体外培养人胆管癌QBC939细胞,噻唑兰比色实验(MTT)观察Celecoxib和5-FU对胆管癌QBC939细胞生长抑制作用;流式细胞术检测细胞生长周期和凋亡率改变。结果:不同浓度的Celecoxib和5-FU可抑制胆管癌QBC939细胞的生长,细胞生长抑制率呈时间-浓度依赖性(P<0.01);实验组QBC939细胞凋亡率随药物浓度的升高逐渐增高(P<0.01),S期细胞逐渐减少(P<0.05),G1期细胞逐渐增加(P<0.05),G2期细胞无明显变化。结论:Celecoxib和5-FU可抑制人胆管癌QBC939细胞的增殖,诱导其凋亡;联合用药效果优于Celecoxib和5-FU单药效果。

Objective：To investigate the effect of Celecoxib and 5-FU on proliferation and apoptosis in human cholangiocarcinoma QBC939 cells line in vitro.Methods：QBC939 cells line were treated with different concentration of Celecoxib or 5-FU,then the inhibition rate of cell growth were detected by MTT and apoptosis index and cell cycle arrest detected by flowcytometer.Results：Different concentration of Celecoxib and 5-FU can inhibit proliferation of QBC939 cells line.The inhibitation effect depends on time and concentration（P〈0.01）;The apoptosis index of QBC939 gradually increase with the increase of drug concentration（P〈0.01）,S stage cell decreases gradually while G1 stage cell increases（P〈0.05）,G2 stage cell ratio has no obvious change.Conclusions ：Celecoxib and 5-FU inhibits proliferation of QBC939 cells line and induce apoptosis in vitro.Combination Celecoxib with 5-FU therapy is better than alone.