摘要
目的:研究APPL1蛋白在人结直肠癌组织中的表达情况与临床病理参数的关系.方法:收集35例新鲜结直肠癌及27例正常直肠黏膜组织,采用免疫组织化学SP法和RT-PCR法检测APPL1在结直肠癌组织及正常直肠黏膜组织中的表达.采用半定量积分分级对该蛋白的表达强弱进行评分.结果:免疫组织化学和RT-PCR结果显示,APPL1蛋白在结直肠癌组织以及正常直肠黏膜组织中普遍表达,但该蛋白和相应的mRNA在癌组织中的表达高于对照组(P<0.05).在35例结直肠癌组织中,APPL1表达与分化程度、淋巴结转移、TNM分期相关(P<0.05),与性别、年龄、肿瘤大小、组织学类型无明显相关(P>0.05).结论:APPL1蛋白在结直肠癌组织中的表达上调,且该蛋白表达与患者肿瘤的分化程度、淋巴结转移情况以及TNM分期有关.APPL1有可能成为结直肠癌治疗的一个新靶点.
AIM：To investigate the relationship between expression of adaptor protein containing PH domain,PTB domain and leucine zipper motif 1（APPL1） and clinicopathological parameters of colorectal cancer.METHODS：Expression of APPL1 protein and mRNA in 35 surgical specimens of colorectal carcinoma （CRC） and 27 normal colorectal tissue specimens was detected by immunohistochemistry and reverse transcription-polymerase chain reaction （RT-PCR）,respectively.RESULTS：The expression of APPL1 in CRC was significantly higher than that in normal mucosa.APPL1 expression was correlated with histological differentiation,lymph node metastasis and TNM stage （all P 0.05）,but not with sex,age,or tumor size （all P 0.05） in patients with CRC.CONCLUSION：The expression of APPL1 protein is increased in CRC.APPL1 protein expression is closely related with tumor differentiation,lymph node metastasis and TNM stage in patients with colorectal cancer.APPL1 may be a novel therapeutic target for CRC.
出处
《世界华人消化杂志》
CAS
北大核心
2012年第3期253-258,共6页
World Chinese Journal of Digestology
