黄芪、三七及其配伍对萎缩性胃炎大鼠胃黏膜热休克蛋白70基因表达的影响

Effect of Astragalus,Panax notoginseng and their compatibility on expression of the heat shock protein 70 gene in rats with atrophic gastritis

目的:阐明黄芪、三七及其配伍对萎缩性胃炎大鼠胃黏膜热休克蛋白70基因表达的影响.方法:健康、清洁级♂Wistar大鼠64只,随机分为空白组9只、假手术组10只,模型组9只,替普瑞酮组9只,黄芪组9只,三七组9只,黄芪+三七组9只.运用幽门弹簧配合高盐热淀粉糊灌胃法制备萎缩性胃炎模型,治疗期共1mo,治疗期间空白组、假手术组、模型组予生理盐水2mL/d灌胃;黄芪组予黄芪水煎剂3.5g/(kg.d)灌胃;三七组予三七粉冲剂0.7g/(kg.d)灌胃;黄芪+三七组予黄芪三七溶液灌胃[其中黄芪3.5g/(kg.d),三七0.7g/(kg.d)];替普瑞酮组治疗期予200mg/(kg.d),治疗结束后,各组取3只,实时定量PCR法测定各组大鼠胃黏膜中Hsp70 mRNA表达量,Western blot法测定Hsp70/Hsp72蛋白表达量,余大鼠HE法观察胃黏膜病理变化.结果:模型组与假手术组相比,其胃黏膜体积构成比明显下降(P<0.01);替普瑞酮组较模型组胃黏膜体积构成比明显升高(P<0.01),黄芪组、三七组和黄芪+三七组与模型组相比,可以明显提高胃黏膜体积构成比(P<0.01),与替普瑞酮比较,差异无统计学差异(P>0.05),黄芪组、三七组、黄芪+三七组之间亦没有统计学差异(P>0.05).黄芪组、三七组胃黏膜中Hsp70 mRNA表达与模型组比较显著升高(P<0.01).各组Hsp70/72蛋白表达差异无统计学意义(P>0.05).结论:黄芪、三七及其配伍可以明显改善萎缩性胃炎大鼠胃黏膜的萎缩状态.诱导热休克蛋白70基因表达可能是黄芪、三七治疗萎缩性胃炎的作用机制之一.

AIM：To evaluate the effect of Astragalus,Panax notoginseng and their compatibility on expression of the heat shock protein 70（Hsp70） gene in rats with atrophic gastritis.METHODS：Sixty-four male healthy Wistar rats were randomized into seven groups：controlgroup（n = 9）,sham operation group（n = 10）,model group（n = 9）,teprenone group（n = 9）,Astragalus group（n = 9）,Panax notoginseng group（n = 9）,and Astragalus plus Panax notoginseng group（n = 9）.Atrophic gastritis was induced by implanting a pylorus spring and intragastrically administering hot salty starch paste.In the one-month therapeutic phase,the control,sham operation and model groups were given normal saline 2 mL daily.The Astragalus group was given water decoction of Astragalus containing crude drug 3.5 g/（kg.d）.The Panax notoginseng group was infused with Panax notoginseng powder containing crude drug 0.7 g/（kg.d）.The Astragalus plus Panax notoginseng group was given both Panax notoginseng powder and Astragalus water decoction.The teprenone group was given teprenone water suspension containing teprenone 200 mg/（kg.d）.All drugs were given by gavage for one month.Three rats of each group were randomly selected for real-time PCR detection of Hsp70 mRNA and Western blot analysis of Hsp70/72 protein.The remaining rats were used for pathological assessment of stomach mucosa by hematoxylin and eosin staining method.RESULTS：Gastric mucosal volume constituent ratio（GMVR） in the model group decreased compared to that in the sham operation group（P 0.01）.The GMVR in the teprenone group increased compared to that in the model group（P 0.01）.The GMVR in the Astragalus,Panax notoginseng,and Astragalus plus Panax notoginseng groups increased compared to the model group,but showed no statistical difference with that in the teprenone group（P 0.05）.Hsp70 mRNA expression in gastric mucosa of rats in the Astragalus group and Panax notoginseng group was higher than that in the model group（both P 0.01）.The expression of Hsp70/72 protein in all groups showed no statistical differences（all P 0.05）.CONCLUSION：Astragalus,Panax and their combination can improve mucosal atrophy in rats with atrophic gastritis.Inducing Hsp70 geneexpression may be one of therapeutic mechanisms for Astragalus and Panax notoginseng in treating atrophic gastritis.