RhoA在胃癌细胞核中的表达及预后意义

Expression and Prognostic Significance of RhoA in Gastric Cancer Cell Nucleus

[目的]探讨RhoA蛋白在胃癌细胞核中的表达及预后意义。[方法]选取有详细临床资料的胃癌组织179例及正常胃黏膜组织80例,应用免疫组织化学改良二步法检测RhoA蛋白的胞核表达。[结果]RhoA蛋白在胃癌及正常胃黏膜组织细胞核中的表达阳性率分别为69.8%(125/179)和25.0%(20/80),差异有显著性(P<0.05)。RhoA蛋白胞核表达率与胃癌肿瘤分化、TNM分期、淋巴结转移有关(P<0.05)。RhoA胞核阳性患者的术后生存期短于阴性患者(P=0.022)。Cox风险比例模型表明,RhoA胞核阳性是胃癌术后预后的独立指标(OR=3.09,P=0.005)。[结论]RhoA蛋白在胃癌细胞核中过表达,是判断胃癌患者术后生存期的独立预后指标。

[Purpose] To investigate the expression of RhoA in gastric cancer cell nucleus and its prognostic significance. [Methods] One hundred and seventy-night gastric cancer tissues and 80 normal gastric tissues with detailed clinical information were enrolled. The expression of RhoA in cell nucleus was detected by Immunohistochemistry （modified two-step method）. [Results] The positive rate of RhoA in gastric cancer and normal mucosa cell nucleus was 69.8% （125/179） and 25.0%（20/80）respectively,with significantly difference （P〈0.05）. The expression of RhoA in gastric cancer cell nucleus was related to differentiation, TNM stage and lymph node metastasis （all P 〈0.05）. The postoperative survival time in gastric cancer patients with positive RhoA expression was significantly shorter than that in patients with negative RhoA expression （P=0.022）.Cox proportional hazards model revealed that RhoA expression was an independent prognostic factor in patients with gastric cancer （OR=3.09,P=0.005）.[Conclusion] RhoA is over expression in gastric cancer cell nucleus, RhoA could serve as an independent prognostic biomarker to evaluate postoperative survival in patients with gastric cancer.