苦参碱对大肠癌细胞凋亡及Bax、Bcl-2表达的影响

The Influence of Matrine on Apoptosis and Expression of Bax and Bcl-2 in Colorectal Cancer Cells

[目的]探讨苦参碱对人大肠癌Lovo细胞增殖抑制和凋亡诱导作用及其对Bax、Bcl-2表达的影响。[方法]0.05~1.6mg/ml不同浓度苦参碱作用Lovo细胞,采用MTT法检测苦参碱对大肠癌Lovo细胞增殖抑制作用,DNAladder、AnnexinⅤ-PI法及TUNEL染色检测细胞凋亡,WesternBlot法检测凋亡相关蛋白Bax、Bcl-2表达的变化。[结果]0.05～1.6mg/ml苦参碱处理Lovo细胞24h或48h后,细胞增殖均明显受抑制;DNAladder、AnnexinⅤ-PI法及TUNEL染色检测结果显示苦参碱呈时间、剂量依赖性诱导细胞凋亡;促凋亡蛋白Bax随着苦参碱剂量增加表达增加,抗凋亡蛋白Bcl-2随着苦参碱剂量增加表达减少。[结论]苦参碱具有抑制大肠癌细胞增殖,诱导其凋亡的作用。苦参碱诱导大肠癌细胞凋亡的机制可能与促凋亡蛋白Bax表达增加、抗凋亡蛋白Bcl-2表达减少有关。

[Purpose] To investigate the effect of Matrine on proliferation inhibition, apoptotic and Bax and Bcl-2 expression in human colorectal cancer cell line Lovo. [Methods] Lovo cells cultured in vitro were interfered with 0.05～1.6mg/ml different concentration of Matrine. The proliferation inhibition effect on Lovo cells was observed by MTT method. Apoptosis induction effect on Lovo cells was detected by DNA ladder, flow cytometer and TUNEL staining. The expression of Bcl-2 and Bax proteins correlated with apoptosis were detected by Western Blot assay. [Results] After being exposed to Matrine （0.05～1.6mg/ml） for 24 and 48h, the proliferation of Lovo cells was inhibited in a dosetime dependent manner. DNA ladder, AnnexinⅤ-PI method and TUNEL staining showed Matrine was obviously increased along with Matrine concentration increased. The expression of pro apoptotic protein Bax was increased, while anti-apoptotic protein Bcl-2 was decreased as Matrine doses increased. [Conclusion] Matrine can inhibit proliferation and induction of apoptosis in colorectal cancer cells. Increased expression of Bax and decreased expression of Bcl-2 might involve in Matrine-induced apoptosis.