补中益气汤对SGC-7901人胃癌细胞株酶活性影响的研究

Effect of Buzhongyiqitang on the activity of Mg^(2+)-ATP and G-6-P enzyme in SGC-7901 human gastric cancer cells

目的研究补中益气汤对人胃癌细胞株酶活性影响,探讨该药作用机制。方法以人胃腺癌细胞株SGC-7901为受试对象,利用血清药理学和酶细胞化学染色法,观察SGC-7901人胃腺癌细胞株的酶活性的改2+变。结果酶细胞化学法显示,中药组细胞质膜标志酶Mg-ATP酶和内质网标志酶G-6-P酶的酶反应颗粒小,数量减少,阳性细胞两种酶的表达量明显低于对照组(<0.05)。结论补中益气汤抗肿瘤作用可能与其降低2+胃癌细胞株Mg-ATP酶和G-6-P酶的活性相关。

Objective To study the effect of Buzhongyiqitang on the activity of Mg2＋-ATP and G-6-P enzyme in SGC-7901 human gastric cancer cells.Method Serum pharmacology method and enzymaticocytochemical staining were used to observe the enzymatic activity change of Mg2＋-ATP and G-6-P in SGC-7901 human gastric gland cancer cell lines.Result Enzyme cytochemical staining showed that the expression levels of plasma membrane marker Mg2＋-ATP enzyme and endoplasmic reticulum marker G-6-P enzyme were lower in the traditional chinese drug group than in the control group and higher than in chemical therapy group（0.05）.Conclusion The anti-tumor role of Buzhongyiqitang might be related to the decrease of Mg2＋-ATP and G-6-P activity.