幼儿牙龈粘膜细胞钙离子浓度与患龋状况关系的研究

Analysis of relationship between gingival cell calcium ions concentration and caries status in children

目的测定幼儿牙龈粘膜细胞内钙离子浓度;探讨幼儿牙龈粘膜细胞内钙离子浓度与患龋状况的关系。方法将50名3~5岁幼儿按患龋状况分为无龋组和龋病高危组。采集幼儿下前牙区唇侧牙龈粘膜脱落细胞,经染色后用激光扫描共聚焦显微镜测定细胞内钙离子的荧光强度,统计分析其与龋患程度的关系。结果高危组幼儿牙龈粘膜细胞的钙离子荧光强度低于无龋组,两组间差异有高度统计学意义;不同性别、不同年龄间牙龈粘膜细胞的钙离子荧光强度无统计学差异;牙龈粘膜细胞钙离子荧光强度值与dft、CSI间分别呈等级负相关关系。结论 3-5岁幼儿牙龈粘膜细胞钙离子浓度与龋患程度有一定相关性,龋患程度重则钙离子浓度低。

Objective To measure the intracellular calcium ions concentration of gingival cells and to discuss the relationship between the calcium ions concentration and caries status in children.Methods Fifty children aged 3~5 year-old were divided into caries free group and high caries-susceptibility group according to caries status.The labial gingival deciduous cells in anterior mandibular zone were collected and died,and then the fluorescent intensity of calcium ions was measured by Laser Scanning Confocal Microscopy.And the relationship between the fluorescent intensity and caries status was statistically analyzed.Results The fluorescent intensity of gingival cell calcium ions in high caries-susceptibility group was statistically lower than that of caries free group.No statistical difference of calcium ions florescent intensities was found among different genders,different ages.The florescent intensity of gingival cell calcium ions was negatively correlated with dft and CSI.Conclusion The calcium ions concentration of gingival cells is related with caries status to some extent in 3~5 aged children.The more serious the caries status is,the lower the calcium ions concentration is.