摘要
目的:建立高效液相色谱法测定肾石通颗粒中槲皮素含量的方法。方法:采用十八烷基硅烷键合硅胶色谱柱(200mm×4.6mm),柱温30℃,流动相为甲醇-水(50∶50),流速1.0mL/min,检测波长360nm。结果:槲皮素在0.0904～0.9040μg范围内质量与峰面积积分值呈良好线性关系,平均回收率98.8%,RSD=1.71%。结论:该法灵敏、准确,可作为肾石通颗粒的质量控制方法。
To develop a method for the content determination of quercetin in Shenshitong Granules by HPLC. Methods： Chromatographic column （200 ram^4.6 mm） of octadecylsilane chemically bonded silica was used. The column temperature was at 30 ~C, the mobile phase was methanol-water （50： 50）,the flow rate was 1.0 mL/min,and the detection wavelength was at 360 nm. Results： There was a good linear relationship between the quality and peak area integral value in the range of 0.090 4~0.904 0 txg for quercetin. The average recovery rate was 98.8% （RSD = 1.71%）. Conclusion： The method is sensitive, accurate and can be used for quality control of Shenshitong Granules.
出处
《中国执业药师》
CAS
2012年第3期17-19,共3页
China Licensed Pharmacist
基金
广东省科技计划项目--以动态逆流提取和纳滤浓缩为核心的高效节能成套技术及装备的开发(2009A030901008)
