摘要
研究了久效磷(Monocrotophos,MCP)对秀丽隐杆线虫(Caenorhabditis elegans)胁迫相关基因mRNA表达的影响。结果表明久效磷暴露能够导致hsp-16.41、hsp-70、cyp-35a2、vit-2、vit-6和age-1基因表达量显著升高,cep-1、ape-1、sod-1、sod-3、ctl-2、ctl-3、gst-1、daf-12和daf-21基因表达量显著降低,对hsp-16.2、mtl-1和mtl-2基因表达量无显著影响。通过主成分分析发现,久效磷主要影响hsp-70、ctl-2、ctl-3、vit-2、cep-1和age-1基因表达,这些基因可作为久效磷毒性效应的敏感分子生物标志物。
Effects of monocrotophos （MCP） on the mRNA expression of stress-related genes of Cae norhabditis elegans were investigated in this research. Treatment with MCP resulted in significant increa- ses of hsp-16.41, hsp-70, cyp-35a2, vit-2, vit-6 and age-1 mRNA expressions, significant decreases of cep-1, ape-l, sod-1, sod-3, ctl-2, ctl-3, gst-1, daf-12 and daf-21 mRNA expressions, and indistinctive changes of hsp-16. 2, mtl-1 and mtl-2 mRNA expressions. Principal component analysis indicated that MCP mainly influences mRNA expressions of hsp-70, ctl-2, ctl-3, vit-2, cep-1 and age-1 genes, which therefore could serve as sensitive molecular biomarkers for the toxicity of MCP.
出处
《中国海洋大学学报（自然科学版）》
CAS
CSCD
北大核心
2012年第3期50-56,共7页
Periodical of Ocean University of China
基金
国家自然科学基金项目(30671618)资助
