白细胞介素4、白细胞介素8、白细胞介素10在哮喘和慢性阻塞性肺疾病发病中的作用

Role of IL-4,IL-8 and IL-10 in Asthma and Chronic Obstructive Pulmonary Disease

目的:探讨外周血白细胞介素4(IL-4)、白细胞介素8(IL-8)、白细胞介素10(IL-10)在哮喘和慢性阻塞性肺疾病(COPD)发病中的作用。方法:采用双抗体夹心酶联免疫吸附法(ELISA)分别检测急性发作期和缓解期哮喘患者各50例(哮喘组)、急性加期和缓解期COPD患者各50例(COPD组)及健康志愿者50例(健康对照组)血清中IL-4、IL-8、IL-10的水平。结果:哮喘组急性发作期IL-4、IL-8水平均显著高于缓解期(P<0.01),且2期均显著高于正常对照组(P<0.01);哮喘组急性发作期IL-10水平显著低于缓解期(P<0.01),且2期均显著低于健康对照组(P<0.01)。COPD组急性加重期IL-4、IL-8水平均显著高于缓解期(P<0.01),且2期均显著高于健康对照组(P<0.01)。哮喘组急性发作期IL-10水平显著低于缓解期(P<0.01),且2期均显著低于健康对照组(P<0.01)。哮喘急性加重期IL-4水平显著高于COPD急性加重期(P<0.01);COPD急性加重期IL-8水平高于哮喘急性发作期(P<0.01);哮喘急性发作期IL-10水平显著低于COPD急性发作期(P<0.01)。结论:IL-4、IL-8、IL-10均参与了哮喘和COPD的气道炎性反应,IL-4、IL-8是促炎因子,而IL-10是抑炎因子。IL-4在哮喘的气道炎症形成中起重要的作用。而IL-8、IL-10为COPD的主要炎症因子。

The research aims at studying the role of peripheral blood interleukin 4 （IL-4）, interleukin 8 （IL-8） and interleukin 10 （IL-10） in asthma and chronic obstructive pulmonary disease （COPD）. Methods： Use double-antibody sandwich enzyme-linked immunosorbent assam （ELISA） to detect the content of serum IL-4, IL-8 and IL-10 in 50 cases of acute exacer- bation of COPD （AECOPD）, 50 cases of paracmastic COPD, 50 cases of acute asthma, 50 cases of paracmastic asthma and 50 cases of healthy volunteers （healthy control group）. Results： The levels of IL-4 and IL-8 in the serum of the patients with acute asthma were significantly higher than that of the paracmastic asthma group（P^0.01）, while the levels of serum IL-4,IL-8 in these two groups were significantly higher than that in the healthy group （P〈0.01）. The levels of IL-10 in the serum of the a- cute asthma group was significantly lower than that in the paracmastic asthma group（P〈0.01 ）, while the level of serum 1I.-10 in both groups were significantly lower than that in the healthy group （P〈0.01）. The levels of IL-4 and IL-8 in the serum of the patients with AECOPD was significantly higher than that in the paracmastic COPD group（P〈0.01）, while the levels of se- rum IL-4 and IL-8 in the two groups were significantly higher than that in the healthy group （P〈 0.01）. the IL-10 level in the serum of the patients with AECOPD was significantly lower than that of the paracmastic COPD group（P〈0.01 ） and the levels of serum IL-10 in both groups were singnificantly lower than in the healthy group （P〈0.01）. The serum IL-4 level in the a- cute asthma group was significantly higher than in the acute asthma group （P〈 0.01）; the serum IL-8 level in AECOPD was significantly higher than in the acute asthma group （P〈 0.01） ; the level of serum IL-10 in the acute asthma group asthma was significantly lower than in AECOPD group （P〈0.01）. Conclusions： IL-4, IL-8 and IL-10 are engaged in asthma and COPD airway inflammatory response. IL-4 and IL-8 are inflammation-promoting factors and IL-10 is inhibitory inflammatory factor. IL-4 plays an important role in asthma＇s airway inflammation and IL-8, IL-10 are main inflammatory factors in COPD.