摘要
目的:探讨外周血白细胞介素4(IL-4)、白细胞介素8(IL-8)、白细胞介素10(IL-10)在哮喘和慢性阻塞性肺疾病(COPD)发病中的作用。方法:采用双抗体夹心酶联免疫吸附法(ELISA)分别检测急性发作期和缓解期哮喘患者各50例(哮喘组)、急性加期和缓解期COPD患者各50例(COPD组)及健康志愿者50例(健康对照组)血清中IL-4、IL-8、IL-10的水平。结果:哮喘组急性发作期IL-4、IL-8水平均显著高于缓解期(P<0.01),且2期均显著高于正常对照组(P<0.01);哮喘组急性发作期IL-10水平显著低于缓解期(P<0.01),且2期均显著低于健康对照组(P<0.01)。COPD组急性加重期IL-4、IL-8水平均显著高于缓解期(P<0.01),且2期均显著高于健康对照组(P<0.01)。哮喘组急性发作期IL-10水平显著低于缓解期(P<0.01),且2期均显著低于健康对照组(P<0.01)。哮喘急性加重期IL-4水平显著高于COPD急性加重期(P<0.01);COPD急性加重期IL-8水平高于哮喘急性发作期(P<0.01);哮喘急性发作期IL-10水平显著低于COPD急性发作期(P<0.01)。结论:IL-4、IL-8、IL-10均参与了哮喘和COPD的气道炎性反应,IL-4、IL-8是促炎因子,而IL-10是抑炎因子。IL-4在哮喘的气道炎症形成中起重要的作用。而IL-8、IL-10为COPD的主要炎症因子。
The research aims at studying the role of peripheral blood interleukin 4 (IL-4), interleukin 8 (IL-8) and interleukin 10 (IL-10) in asthma and chronic obstructive pulmonary disease (COPD). Methods: Use double-antibody sandwich enzyme-linked immunosorbent assam (ELISA) to detect the content of serum IL-4, IL-8 and IL-10 in 50 cases of acute exacer- bation of COPD (AECOPD), 50 cases of paracmastic COPD, 50 cases of acute asthma, 50 cases of paracmastic asthma and 50 cases of healthy volunteers (healthy control group). Results: The levels of IL-4 and IL-8 in the serum of the patients with acute asthma were significantly higher than that of the paracmastic asthma group(P^0.01), while the levels of serum IL-4,IL-8 in these two groups were significantly higher than that in the healthy group (P〈0.01). The levels of IL-10 in the serum of the a- cute asthma group was significantly lower than that in the paracmastic asthma group(P〈0.01 ), while the level of serum 1I.-10 in both groups were significantly lower than that in the healthy group (P〈0.01). The levels of IL-4 and IL-8 in the serum of the patients with AECOPD was significantly higher than that in the paracmastic COPD group(P〈0.01), while the levels of se- rum IL-4 and IL-8 in the two groups were significantly higher than that in the healthy group (P〈 0.01). the IL-10 level in the serum of the patients with AECOPD was significantly lower than that of the paracmastic COPD group(P〈0.01 ) and the levels of serum IL-10 in both groups were singnificantly lower than in the healthy group (P〈0.01). The serum IL-4 level in the a- cute asthma group was significantly higher than in the acute asthma group (P〈 0.01); the serum IL-8 level in AECOPD was significantly higher than in the acute asthma group (P〈 0.01) ; the level of serum IL-10 in the acute asthma group asthma was significantly lower than in AECOPD group (P〈0.01). Conclusions: IL-4, IL-8 and IL-10 are engaged in asthma and COPD airway inflammatory response. IL-4 and IL-8 are inflammation-promoting factors and IL-10 is inhibitory inflammatory factor. IL-4 plays an important role in asthma's airway inflammation and IL-8, IL-10 are main inflammatory factors in COPD.
出处
《中国临床医学》
2012年第1期22-24,共3页
Chinese Journal of Clinical Medicine