周期蛋白D1在先天性与后天性胆脂瘤中的表达

Expression of cyclinD1 in congenital and acquired cholesteatoma

目的通过研究周期蛋白D1(CyclinD1)在先天性胆脂瘤和后天性胆脂瘤的表达,探讨其表达差异的意义。方法选取我院就诊的60例中耳胆脂瘤患者,其中先天性胆脂瘤25例,后天性胆脂瘤35例;同时选取同期3例慢性中耳炎手术患者正常耳廓后皮肤组织作为对照组。采用免疫组化方法检测胆脂瘤组织中CyclinD1的表达。结果 CyclinD1免疫组化染色在先天性胆脂瘤和后天性胆脂瘤中均为阳性表达,但后天性胆脂瘤中阳性表达率高于先天性胆脂瘤,其差异具有统计学意义(P<0.01);CyclinD1在正常皮肤中不表达。结论 CyclinD1在后天性胆脂瘤上皮表达增强,可能是炎症刺激所致。

To study the expression d cyclinD1 in congenital and acqnirexl cholesteatoma and its signLqcance. Methods 25 samples of congenital cholesteatoma, 35 of acquired eholesteatoma and 3 of normal auricle skin in the patients of chronic otitis media were included. The expression of cyclinD 1 in these samples was detected with immunohistochemical method. Results CyelinD1 was positively expressed in cholesteatoma, but not in normal skin. As in the cholesteatoma, the positive rate of cycl/nD 1 in acquired ones was significantly higher than that in the congenital and the difference was statistically significant （ P 〈 0.01 ）. Conclusion Acquired cholesteatoma has stronger proliferation ability than congenital cholesteatoma, which may be caused by inflammatory stimuli.