骨桥蛋白在不同侵袭转移潜能葡萄膜黑色素瘤中的表达及意义

Expression of osteopontin in different metastatic potential uveal melanoma and its significance

背景骨桥蛋白（OPN）在多种转移性肿瘤组织中表达增高，但在葡萄膜黑色素瘤（UM）中的表达与临床病理特征及侵袭转移是否相关尚不清楚。目的研究UM组织及不同转移潜能人15M细胞系MUM一2B、C918和OCM一1A中OPN的表达情况，分析OPN的组织及细胞系表达水平与UM患者临床病理特征与转移预后之间的关系。方法收集2004年1月至2007年12月在北京同仁医院行眼球摘除手术并已经病理证实为脉络膜黑色素瘤组织的标本共50例，应用免疫组织化学法检测石蜡标本中OPN的表达，分析其与临床资料的相关性。应用定量聚合酶链反应（Q—PCR）技术检测不同侵袭性转移潜能的人UM细胞系中OPNmRNA的表达情况。结果50例uM组织中发生肝转移者13例，其中10例OPN表达阳性，未发生肝转移的37例中14例OPN表达阳性；上皮型与非上皮型脉络膜黑色素瘤OPN表达阳性者分别为11／15和13／35；肿瘤累及睫状体和未累及者OPN表达阳性者分别为20／30和4／20；上述指标的比较差异均有统计学意义（，=5．888、5．510、10．470，P〈0．05）。患者不同性别、年龄、眼别、肿瘤最大基底径以及是否侵犯巩膜导管间OPN表达阳性例数的比较差异均无统计学意义（P=0．536、0．256、0．802、0．848、0．555）。转移潜能细胞系由高到低依次为MUM一2B、C918、OCM一1A，其OPNmRNA相对表达量分别为1．00±0．04、0．91±0．03、0．08±0．01，差异有统计学意义（F=33．135，P〈0．05），MUM一2B、C918中OPNmRNA的表达水平较OCM一1A中明显增高，差异均有统计学意义（P=0．00），而MUM一2B、C918中OPNmlq．NA的表达水平差异无统计学意义（P：0．804）。结论OPN与UM侵袭能力及转移潜能密切相关，发生转移的UM组织及高侵袭转移性细胞系中OPN表达水平明显升高，提示OPN可能作为预测UM侵袭能力、转移潜能以及患者预后的指标。

Background Osteopontin is highly expressed in many metastatic tumors, but its expression in uveal melanoma and the relationship of osteopontin with metastasis is still under investigation. Objective This study was to investigate the relationship between osteopontin expression and uveal melanoma metastatic potential. Methods Fifty samples of uveal melanoma were collected in Beijing Tongren Hospital. The expressions of osteopontin in the samples were detected using immunohistoehemistry, and expressions of osteopontin mRNA in different metastatic potential Uveai melanoma cell lines （MUM-2B,C918,0CM-1A） were analyzed using quantitative polymerase chain reaction （Q-PCR）. Results Osteopontin was positively expressed in 10 samples of 13 patients with metastasis and 14 samples of 37 patients without metastasis, showing a significant difference between them （X2= 5. 888,P= 0. 000）. There were 11 samples and 13 samples exhibiting the positive expression of osteopontin in 15 epithelial type and 35 non-epithellal type ~f uveal melanoma respectively with the significant difference （X2 = 5. 510, P = 0. 000）. The positive expressing numbers of osteopontin were 20 tumors of 30 affected tumors and 4 tumors of 20 unaffected ciliary tumors with the significant difference （X2 = 10. 470, P = 0. 000）. No significant differences in the expression of osteopontige were found between different gender, age, eyes, largest basal diameters and invasion of scleral canal（P= 0. 536,0. 256,0. 802,0. 848,0. 555 ）. The relative expression value of osteopontin mRNA was in turn 1.00±0. 04,0. 91 ±0. 03,0. 08±0.01 in MUM-2B, C918, OCM-1A with the significant difference among three cell lines（ F= 33. 135 ,P〈0.05 ）. The osteopontige mRNA expression was significant enhanced in high metastatic potentialcell lines （MUM-2B, C918 ） compared with low metastatic potential cell lines （OCM-1 A） （P〈0.05）, but no significant difference was seen between two high metastatic potential cell lines（ MUM-2B, C918 ） （P = 0. 804 ）. Conclusions Osteopontige expression is associated with the uveal melanoma cell line invasion and metastasis potential. These results imply that osteopontige expression maybe an indicator of uveal melanoma invasion and metastasis ability.