摘要
为提高水稻品种抗性,以特种稻龙稻8号和龙稻9号的成熟种子作为试验材料诱导形成胚性愈伤,分别用携带有pCDMAR-UBb-Hyg质粒单价Cry1Ab基因的菌株LBA4404和携带有pCDMAR-UBAC-Hyg质粒双价Cry1Ac+SCK基因的菌株LBA4404为Bt基因的载体,再以农杆菌介导的方法实现Bt基因对龙稻8号和龙稻9号基因座的修饰。结果表明:两种质粒的转化率都在20%～30%,而两个品种的Bt蛋白表达阳性几率存在一定的差异。
In order to enhance resistance of rice, bacterial strain LBA4404 which contained two different types of plasmid pCDMAR-UBb-Hyg and pCDMAR-UBAC-Hyg modified CrylAb and CrylAC＋SCK separately were used as mediator to carry out locus modifying of I.ongdao No. 8 and Longdao No. 9. The results showed that conversion percentage of both the two plasmids were between 20 % and 30 %. But percentage of positive test for Bt protein showed a little difference.
出处
《黑龙江农业科学》
2012年第3期22-25,共4页
Heilongjiang Agricultural Sciences
基金
国家转基因专项资助项目(2008ZX08001-001)
黑龙江省自然科学基金重点资助项目(2009HSJ-E-2)
哈尔滨市青年基金资助项目(92007RFQYN106)
关键词
水稻
BT基因
转化率
分化率
rice
Bt gene
conversion percentage
differentiation rate
