摘要
为提高粳稻抗性愈伤分化率和遗传转化率,以农杆菌介导遗传转化得到的水稻成熟胚抗性愈伤(Hyg)为材料,研究了影响粳稻成熟胚抗性愈伤组织再生频率的因素。结果表明分化培养基添加0.5～25 mg.L-1的CuSO4.5H2O,增加分化培养基中的琼脂浓度,分化前对抗性愈伤进行高渗处理,采用6-BA和KT两步分化法和干燥除菌法均能提高抗性愈伤组织的分化率。
To improve differentiation and transformation ratio,resistant calli（Hyg） in mature embryo of Japonica rice,obtained through genetic transformation by Agrobacterium associated method,were used to study the influence factors of regeneration frequency. The results indicated that supplement with 0.5～25 mg·L-1 CuSO4·5H2O and strengthening agar concentration in differentiation medium could improve significantly differentiation rate of resistant calli from Japonica rice,as well as other measures including high osmotic pressure treatment of calli,the two-step regeneration with 6-BA and KT in turn and the dessication treatment to eliminate Agrobacterium.
出处
《黑龙江八一农垦大学学报》
2012年第1期28-31,共4页
journal of heilongjiang bayi agricultural university
关键词
抗性愈伤组织
再生
水稻
高渗处理
铜元素
resistant callus
regeneration
rice
high osmotic pressure treatment
copper
