利用木聚糖酶酶解小麦麸皮制备低聚木糖工艺参数的研究

Study on Preparation of Xylooligosaccharides from Wheat Bran

研究利用木聚糖酶酶解小麦麸皮制备低聚木糖。采用正交旋转组合实验优化设计,确定木聚糖酶酶解制备小麦麸皮低聚木糖的最佳工艺参数。结果表明,底物浓度为10.5%,加入酶量为1 000 IU/g底物,水解温度为53℃,水解时间为5.5 h,最终得到酶解液中低聚木糖的平均聚合度为2.18,总还原糖含量为5.83 mg/mL。并通过HPLC分析确定酶解液中主要含有木二糖、木三糖、木四糖、木五糖等低聚木糖组分,且低聚木糖(木二～木五)的相对含量达64.41%。说明此水解条件能够较好的制备低聚木糖。

The study is using xylanase to enzymic hydrolysis xylan for preparating xylo-oligosaccharide.The orthogonal rotation group practices is used,and the best technological parameter is definited on xylanase to enzymic hydrolysis xylan for preparating xylooligosaccharide. The result shows that concentration of substrate is 10.5%,adding enzyme is 1 000 IU/g,hydrolysis temperature is 53 ℃,hydrolysis time is 5 h,the DP is 2.18.Though the HPLC,the enzymolysis liquid has disaccharide,trisaccharide,tetrose,pentosaccharide, and relative amount is 64.41%.This hydrolyse condition can prepare xylooligosaccharides very well.