野生仙人掌多糖对小鼠特异性免疫功能的调节作用

Immunomodulatory Effects of Opuntia dillenii Polysaccharides on Specific Immune Function of Mice

目的:观察野生仙人掌多糖(ODPs)对小鼠特异性(体液及细胞)免疫功能的调节作用。方法:采用环磷酰胺建立免疫抑制小鼠模型,检测ODPs对血清溶血素IgMI、gG含量、脾淋巴细胞增殖反应和外周血T淋巴细胞亚群比例的影响;检测分离纯化的ODPs主要组分ODP-I对体外培养的淋巴细胞增殖作用。结果:ODPs能显著增强免疫抑制小鼠血清溶血素IgMI、gG的生成;显著增强免疫抑制小鼠T、B淋巴细胞的增殖能力,且均使之恢复至正常水平;显著降低免疫抑制小鼠CD4+T细胞亚群比例,从而显著降低CD4+/CD8+T细胞比值,使之接近正常水平,且均有剂量关系。ODP-I可极显著促进小鼠脾淋巴细胞自然增殖,协同有丝分裂原ConA或LPS诱导T、B淋巴细胞的增殖。结论:ODPs对免疫抑制小鼠特异性免疫功能具有调节作用,对体外淋巴细胞具有增殖作用,是一种良好的免疫调节剂。

Objective：To investigate specific immune modulation of Opuntia dillenii polysaccharides （ODPs）on mice （body fluid and cells）. Methods：An immunosuppressed murine model was induced by intraperitoneal injection of cyclophosphamide （Cy, ip）. The intraserous hemolysin IgM, IgG levels, the proliferation of spenocytes and the proportion of T lymphocyte subsets in peripheral blood were determined after the mice treated by ODPs. Proliferative effects of ODP-I, the main component purified from ODPs, on the lympho- cytes in vitro were also studied. Results：ODPs could significantly increase intraserous IgM and IgG levels, significantly enhance the proliferation of T and B lymphocytes and restore them to normal level. ODPs significantly reduced the percentage of CIM ＋ T lympho- cytes subset, therefore significantly reduced the proportion of CIM ~ and CD8 ~ T lymphocytes of peripheral blood from immunosuppres- sive mice and restored it to normal level. ODP-I significantly enhanced the natural proliferation of splenic lymphocyte and assisted the proliferation of T and B lymphocytes which induced by mitogen ConA or LPS. Conclusion ： ODPs can enhance the specific immune func- tion of immunosuppressed mice and the proliferation of lvmphocvtes in vitro.