大黄虫丸抗动脉血栓的正交实验研究

Study of Dahuangzhechong Pills on Anti-arterial Thrombosis with the Orthogonal Design

目的:采用正交设计筛选大黄虫丸抗动脉血栓形成的主要成分,精制大黄虫丸。方法:将单味药材按照正交设计表(L16215)依次配伍,分为16组并制成相应药液,予SD大鼠灌胃,模型组、正常对照组给予等体积生理盐水灌胃。灌胃1 w后分离大鼠颈动脉,按三氯化铁诱导大鼠颈总动脉血栓模型方法造模,造模后取血,检测血小板、血栓素B2(throm boxaneB2,TXB2)和6酮前列腺素F1α(6-keto-prostaglandin F1α,6-keto-PGF1α),取血后剪取大鼠造模处动脉烘干称量质量后动脉放入10%福尔马林溶液中固定,HE染色,观察血管组织形态学改变。结果:病理切片显示各实验组均有血栓形成,但血栓形成严重程度不一,出现软化、溶解、吸收,内膜损伤。正交分析结果显示:1、对血栓干质量有影响的是:大黄、土鳖虫、水蛭、桃仁、干漆,其中除干漆P<0.05外,其他均P<0.01。2、对血浆6-keto-PGF1α水平有影响的是:桃仁、干漆、土鳖虫、虻虫、蛴螬、水蛭、大黄,其中除大黄P<0.05外,其他均P<0.01。3、对血浆TXB2水平有影响的是:土鳖虫、桃仁、干漆、大黄、水蛭,其中除水蛭P<0.05外,其他均P<0.01。4、对血小板计数有影响的是:桃仁、干漆、大黄、土鳖虫、虻虫、水蛭,其中除虻虫、水蛭P<0.05外,其他均P<0.01。结论:大黄虫丸抗动脉血栓作用与大黄、土鳖虫、水蛭、桃仁、干漆、虻虫6味药材关系最为密切。

Objective：To screen the main component of Dahuangzhechong pill＇s anti-arterial thrombosis with the orthogonal design and refine Dahuangzhechong pills. Methods：In accordance with the orthogonal design table（ Lt62-5）, divided herbs into 16 groups and made the appropriate liquid. Tile liquid was gave to SD rats by intragastric administration, the model group, normal control group re- ceived the same volume of physiological saline. Isolated rats＇ carotid artery after intragastric administration a week, modeled according to ferric chloride inducement the carotid artery thrombosis method, then collected blood, detected content of platelet, thromboxane B2 （ TXB2 ） and 6-keto-prostaglandin F1α （ 6-keto-PGF1α ）, sheared and measured dry weight of the modeling artery, then placed arteries in 10% formalin fixation, observed morphological changes in vascular tissue by HE staining. Results： Pathological examination revealed： each experimental group had thrombosis, softening, dissolution, absorption, and intimal injury, but the severity of thrombosis were dire- rent. Orthogonal analysis showed ： 1, influence on dry weight of thrombus ： rhubarb, ground beetle, leeches, peach seed, dry paint, except dry paint P 〈 0. 05, the others P 〈 O. 01. 2, influence on plasma 6- keto-PGF1α level ： peach seed ,dry paint, ground beetle, gadfly, grubs, leeches, rhubarb, except rhubarb P 〈 0. 05, the others P 〈 O. 01. 3, influence on plasma TXB2 ： ground beetle, peach seed, dried paint, rhubarb, leeches, except leech P 〈 O. 05, the others P 〈 O. 01. 4, influence on platelet count ： peach seed, dry paint, rhubarb, ground bee- tle, gadfly, leeches, except gadfly,leeches P 〈 O. 05, the others P 〈 0. 01. Conclusion ： Anti-artery thrombosis of Dahuangzhechong Pill is most closely related with rhubarb, ground beetle, leeches, peach seed, dry paint and gadfly.