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锂剂和氯胺酮对N2a细胞mTOR及GSK-3β的影响 被引量：1

Effects of lithium and ketamine on the expression of mTOR and GSK-3β in N2a cells
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摘要 目的观察锂剂和氯胺酮分别对N2a细胞中西罗莫司靶蛋白（mTOR）及糖原合酶激酶-3β（GSK-36）的影响。方法将N2a细胞随机分为3组（n=6），分别加入DMEM培养液（C组）、3μmol／L氯化锂（L组），1μmol／L氟胺酮（K组），孵育2d后采用WesternBlotting分别检测mTOR和GSK-3β表达。结果与C组相比，L组和K组mTOR表达显著上调，GsK-3β表达显著下调（P〈0．05）。结论锂剂和氯胺酮可上调mTOR，下调GSK-3β。 Objective To evaluate the effects of lithium and ketamine on the expression of mammalian target of rapamycin （mTOR） and glycogen synthase kinase-3 beta （GSK-3β） in N2a cells. Methods N2a cells were randomly divided into 3 groups （n = 6） . DMEM culture solution （group C）, lithium chloride 3/Lmol/L（group L） and ketamine 1 μmol/L（group K）. Western Blotting was used to determine the expression of mTOR and GSK-3β in N2a cells 2 d after incubation. Results Compared with group C,group L and group K both had up-regulated mTOR and down-regulated GSK-3β （P 〈 0. 05）. Conclusions lithium and ketamine could mediate the up-regulation of roTOR and down-regulation of GSK-3β.
作者 石金云 李文媛 杨春 杨建军 周志强 徐建国
机构地区 南京大学医学院临床学院南京军区南京总医院麻醉科 南京大学医学院附属鼓楼医院麻醉科
出处 《神经疾病与精神卫生》 2012年第1期12-13,共2页 Journal of Neuroscience and Mental Health
基金 国家自然科学基金（30872424）资助 全军“十二五”科研面上项目资助
关键词 锂 氯胺酮 西罗莫司靶蛋白 糖原合成酶激酶-3Β N2A细胞 Lithium Ketamine,Sirolimus, Glycogen synthase kinase-3β
分类号 R734.2 [医药卫生—肿瘤]
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参考文献8

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同被引文献13

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  • 7Raedler TJ. Inflammatory mechanisms in major depressive dis- order[J]. Curr Opin Psychiatry,2011,24(6) :519-525.
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神经疾病与精神卫生
2012年 第1期

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