摘要
采用α-淀粉酶水解提取紫甘薯花色苷色素,研究了反应温度、时间、pH值以及酶与底物比对提取效果的影响,结果表明最佳提取条件为:反应温度60℃,pH值5.5,反应时间70 min,酶与底物比400 U/mL。采用大孔树脂吸附法对色素提取液进行纯化,比较发现4种大孔树脂中AB-8型大孔树脂的吸附效果最好,并进一步研究了紫甘薯花色苷色素在AB-8型大孔树脂上的动态吸附及解吸过程。
In this paper,anthocyanins pigment is extracted by α-amylase from purple sweet potato,and refined by macroporous resin.The influence of different reaction conditions are investigated,the result shows that the optimal extractive conditions are： reaction temperature 60 ℃,pH value 5.5,reaction time 70 min,enzyme-substrate ration（E/S） 400 U/mL.The purification effect of AB-8 is better than other three kinds of macroporous resin,then further studies the dynamic adsorption and desorption process.
出处
《农产品加工（下）》
2012年第3期64-66,70,共4页
Farm Products Processing
关键词
紫甘薯花色苷色素
Α-淀粉酶
大孔树脂
anthocyanins pigment from purple sweet potato
or-amylase
macroporous resin
