CHU-R菌株产虾青素的发酵工艺研究

Fermentation of CHU-R for Astaxanthin Production

本文通过对获得的虾青素产生菌CHU-R进行最佳培养基、培养温度、接种量、初始pH等发酵工艺参数的优化研究，得到CHU-R菌产虾青素的最佳培养基为：6％蔗糖、1％糖蜜、0．5％硫酸铵、0．3％NazHPO4、0．05％KI、0．05％MgSO4·7H2O；50L罐最适发酵工艺条件为：温度28℃，通风量1：0．1加．15（V／V／min）、初始转速120r／min、罐压0．054）．08MPa、装液量30L、初始pH6．8-7．0、接种量6％，发酵过程维持溶氧量在40-60％之间。由此得到的虾青素含量近达23mg／g干菌体重。

The fermentation process parameters of CHU-R, such as medium, incubation temperature, inoculation, etc. were discussed in this paper. It was found that the best medium formulas was： 6% sucrose, 1% molasses, 0.5% ammonium sulfate, 0.3% Na2HPO4, 0.05% KI, and 0.05% MgSO4·7H2O. The optimum fermentation conditions in 50 L fermenter were： temperature 28℃, ventilation 0.1--0.15 （V/V/min）, the initial speed of 120rpm, tank pressure 0.05-0.08 MPa, liquid volume 30 L, the initial pH 6.8-7.0 and inoculum size 6%, the fermentation process to maintain dissolved oxygen at 40-60%. The results showed that the CHU-R yield pigment nearly reached 23 mg/g of its dry weigh fermented in 50 L fermenter.