大高良姜的化学成分及对人乳腺癌细胞MCF-7的细胞毒作用研究

Study on Chemical Constituents of Alpinia Galanga Rhizomes and Their Cytotoxicity in MCF-7 Cells

目的研究大高良姜的化学成分及所得部分苯丙素类化合物对人乳腺癌细胞MCF-7的细胞毒活性。方法用柱色谱技术分离、纯化,通过理化常数和波谱分析鉴定化合物结构;细胞培养MTT染色法测定化合物对肿瘤细胞的毒性。结果分离并鉴定出10个化合物,分别为:二乙酸-反式-对香豆醇酯(Ⅰ),对乙酰氧基苯内烯醇(Ⅱ),对乙酰氧基苯丙烯醚(Ⅲ),1-乙酰氧基胡椒酚乙酸酯(Ⅳ),反式对羟基桂皮醛(Ⅴ),反式对羟基桂皮酸(Ⅵ),反式-对羟基桂皮醛乙酯(Ⅶ),二对乙酰氧基苯乙烯甲烷(Ⅷ),对羟基苯甲醛(Ⅸ),2,5-二羟基苯甲酸(Ⅹ);化合物Ⅰ对人乳腺癌细胞MCF-7生长抑制作用较强,化合物Ⅱ～Ⅵ无明显细胞毒作用。结论化合物Ⅱ,Ⅹ系首次从该植物中分离到;化合物Ⅰ对人乳腺癌细胞MCF-7有一定的细胞毒作用。

Objective To study the chemical constituents ofAlpinia galanga rhizomes and their cytotoxicity in MCF-7 cells. Methods The chemical constituents were isolated and purified by Column Chromatography and their structures were identified on the basis of physicochemical properties and spectral analysis. The MTT method was applied to examine the growth inhibition of MCF-7 cells treated with these compounds. Results Ten compounds were isolated and identified as trans-p-coumaryl diacetate（ I ）, （E）-3-（4-acetyloxyphenyl）propen-l-ol（ II ）, 4,4＇-[（2E,2＇E）-bis-（prop-2- ene）-l,l＇-oxy]-diphenyl-7,7＇-diacetate（III）, l＇-acetoxychaviol acetate（IV）, trans-p-hydroxycinnamaldehyde（V）, trans- p-hydroxycinnamic acid（VI）, trans-p-hydroxylcinnamaldehyde acetate（VII）, [di-p-acetoxy-cis-styryl]methane （VIII）, p-hydroxybenzaldehyde （IX）, 2,5-dihydroxybenzoic acid（X）. Compound I could inhibit the proliferation of MCF-7 significantly, but compounds II-VI almost did not have inhibition to MCF-7 cells. Conclusion The compounds of II, X can be separated from this plant for the first time. Compound I shows cytotoxic activities on MCF-7 cells.