沙鼠肝泡球蚴组织中骨桥蛋白与IL-10的表达及相关性研究

Expressions of osteopontin and interleukin-10 in Gerbil hepatic alveolar hydatid tissue and their correlation

目的检测沙鼠肝泡状棘球蚴组织中骨桥蛋白(OPN)及白细胞介素-10(IL-10)的表达,并进一步探讨两者的相关性。方法 20%泡状棘球蚴组织混悬液开腹直视下肝脏穿刺注射感染长爪沙鼠60只,每只0.1mL,建立肝泡状棘球蚴长爪沙鼠模型。随机分为模型组(无干预,40只)和实验组(抗OPN抗体干预,20只)。感染至100d时剖杀所有长爪沙鼠,观察泡状棘球蚴生长和转移情况,取长爪沙鼠肝泡状棘球蚴组织和转移灶标本。采用免疫组织化学SP法观察沙鼠肝泡状棘球蚴组织和转移灶中OPN及IL-10的表达情况。结果 60只长爪沙鼠全部感染肝泡状棘球蚴。免疫组化染色显示肝泡状棘球蚴组织可见OPN及IL-10不同程度的表达。模型组中OPN和IL-10阳性细胞表达率分别为72.5%(29/40)和65%(26/40),OPN及IL-10主要分布在肝泡状棘球蚴纤维囊壁,阳性产物均定位于细胞浆。65%(26/40)的长爪沙鼠模型经病理证实发生胸廓内淋巴结转移,伴有胸廓淋巴结转移的肝泡状棘球蚴组织中OPN及IL-10阳性细胞表达率分别为84.6%(22/26)、76.9%(20/26),均高于未发生胸廓淋巴结转移的肝泡状棘球蚴组织(50%、42.9%)(P<0.05)。实验组OPN和IL-10的阳性细胞表达率分别为40%(8/20)和35%(7/20)。OPN与IL-10表达呈正相关(r=0.605,P=0.000)。结论 OPN阳性表达可能与肝泡状棘球蚴的转移有关,OPN与IL-10可能在肝泡状棘球蚴的生长中起协同作用。

To investigate the expressions of osteopontin and interleukin-10 and their correlation in Gerbil hepatic alveolar hydatid tissue,60 gerbils were infected with 20% E.multilocularis suspension（0.1 mL per gerbil） through abdominal opening injection in liver.They were randomly divided into model group（No intervention,40 gerbils） and experimental group（Anti-OPN antibody interference,20 gerbils）.Gerbils were sacrificed at 100 days post-infection for observing the growth and metastasis of hepatic echinococcus cyst.The expressions of OPN and IL-10 in hepatic echinococcus cyst and metastasis tissue were measured by immunohistochemistry staining（SP method）.Results showed that all gerbils infected with hepatic alveolar hydatid.expression of OPN and IL-10 were found at different degree in echinococcus cysts.In model group,the positive expression rate of OPN and IL-10 in echinococcus cysts were 72.5%（29/40） and 65%（26/40）.Both OPN and IL-10 were mainly distributed in the germinal layer.The 65%（26/40） of them occurred thoracic lymph node metastases.The OPN and IL-10 expression rate in hepatic echinococcus cysts with thoracic lymph node metastases were 84.6%（22/26） and 76.9%（20/26）,which were significantly higher than that of hepatic echinococcus cysts without thoracic lymph node metastases（50%,42.9%）（P0.05）.In experimental group,the positive expression rates of OPN and IL-10 in echinococcus cysts were 40%（8/20） and 35%（7/20）.The expression of OPN was significantly correlated with the expression of IL-10（r=0.605,P=0.000）.In conclusion,OPN might significantly relate to the metastasis of hepatic echinococcus cysts.OPN and IL-10 might play synergetic roles in hepatic alveolar hydatid tissue.