余甘子对大鼠非酒精性脂肪肝疾病中肝损伤和炎症的抑制作用研究

Amelioration of Hepatic Injury and Inflammation in Rat Non-alcoholic Fatty Liver Disease Model by Phyllanthus Emblica

目的探讨余甘子对大鼠非酒精性脂肪肝模型中肝损伤和炎症的抑制作用。方法将健康雌性SD大鼠21只随机分为3组。对照组大鼠以普通饲料喂饲,非酒精性脂肪肝组大鼠喂以添加10%的猪油以及2%胆固醇的高脂饲料。余甘子处理组大鼠的饲料与非酒精性脂肪肝组大鼠相同,另外每日每只大鼠注射0.06ml/g的余甘子萃取物。8周后取大鼠血清和肝脏进行检测。结果高脂饲料喂养8周后,非酒精性脂肪肝组大鼠血清中谷丙氨酸转氨酶(ALT)和天门冬氨酸转氨酶(AST)显著上升,肝脏组织切片中出现明显的炎症位点和脂肪累积,肝脏中炎症因子(TNF-α、IL-1β、COX-2)的表达量亦显著上升。加入余甘子处理的非酒精性脂肪肝大鼠以上指标均有明显改善。结论余甘子可改善非酒精性脂肪肝形成过程中肝脏的损伤和炎症的发生。

Objective To study the ameliorative effect of phyllanthus emblica on hepatic injury and inflammation in rat non-alcoholic fatty liver disease（NAFLD） model.Methods Twenty one healthy female SD rat were randomly divided into 3 groups.Control group was fed with regular chow.NAFLD group was fed with high-fat diet（chow supplemented with 10% oil and 2% cholesterol）.Phyllanthus emblica co-treated group was fed with high-fat diet accompanied by daily i.p.injection of 0.06 ml/g extract of phyllanthus emblica.Eight weeks later,serum and liver samples from rats were subjected to measurements.Results After 8-week high-fat diet feeding,serum levels of ALT and AST in NAFLD rats were significantly increased.Obvious inflammatory site and fat accumulation were observed in NAFLD rats with up-regulation of hepatic pro-inflammatory factors.Co-treatment of phyllanthus emblica effectively attenuated fatty liver pathological features.Conclusion Phyllanthus emblica can ameliorate hepatic injury and inflammation during NAFLD.