食用骨粉对大鼠致畸作用的研究

A Study of the Teratogenicity of Edible Bone Powder in Rats

目的探讨食用骨粉对大鼠的致畸作用。方法采用大鼠致畸试验,将孕鼠随机分为5组,实验组剂量分别为0.25g/kg(体重)、2.5g/kg(体重)、5g/kg(体重),阴性对照组给予等量去离子水。在孕7～16d,各剂量组及阴性对照组均按1.5ml/100g(体重)经口灌胃,阳性对照组于孕12d腹腔注射环磷酰胺1.0ml/100g(体重)。孕21d处死孕鼠,检查母体妊娠与胎鼠畸形情况。结果各实验组母鼠体重、胚胎毒性、致畸毒性与阴性对照组比,差异无统计学意义(P>0.05)。结论食用骨粉在0.25～5g/kg(体重)剂量下未显示致畸作用。

Objective To explore the teratogenicity of edible bone powder in rats. Methods The samples were tested with teratogenicity test. Pregnant rats were divided into 5 groups including expose groups and control groups. Different doses of edible bone powder （0. 25,2.5,5 g/kg） and deionized water were gavaged to expose groups and negative control group from 7th to 16th day of gestation respectively, and cyclophosphamide was intraperitoneal injected to positive control group at 12th day of gestation. Pregnant rats were killed and examined at 21th day of gestation. Results No significant differences were observed in the weight of pregnant rats, fetal and teratogenic toxicity between the expose and control groups. Conclusion Teratogenicity of edible bone powder at 0. 25 - 5 g/kg was not found in rats.