慢性砷中毒大鼠肾脏蛋白激酶C-α达的研究

Protein kinase C-α expression in kidney of rat with chronic arsenic poisoning

【摘要】目的探讨蛋白激酶C（PKC）-α在慢性砷中毒大鼠肾脏的表达及作用。方法将60只清洁级SD大鼠按体质量随机分为高砷（10．0mg／kg）、低砷（0．4mg／kg）组和对照（蒸馏水）组，采用自由饮水方式进行染毒，每10天称体质量1次，调节给药量。连续染毒4个月，测血砷、尿砷。取大鼠肾脏用免疫组织化学SABC法，观察肾脏PKC-α阳性细胞并计数，采用Biomias图像分析软件测定其平均灰度值。结果高砷组和低砷组的SD大鼠肾脏近端小管PKC-α阳性细胞计数[（3．62±1．90）、（10．07±3．22）个／视野]、肾小球PKC-α阳性细胞计数[（3．62±1．90）、（10．07±3．22）个／视野]均低于对照组[（60．00±9．63）、（18．57±2．71）个／视野，P均〈O．05），高砷组和低砷组的大鼠尿砷[（7366．624±1086．50）、（1744．314±300．12）μg／L]、血砷[（31．59±9．24）、（16．58±2．08）pr,／L]均高于对照组[（18．97±3．58）、（18．974±3．58）lxg／L，P均〈0．05]。高砷组和低砷组的SD大鼠肾脏近端小管、肾小球PKC-α阳性细胞平均灰度值（142．79±11．16、122．15±5．91）均高于对照组（114．33±6．70，P均〈0．05）。结论砷可使SD大鼠肾脏PKC-α阳性细胞表达减少。

Objective To investigate the expression and relevant function of protein kinase C （PKC）-β in kidney of rat with chronic arsenic poisoning. Methods Totally 60 healthy SD rats of clean grade were randomly divided by body weight into 3 groups： high-dose arsenic exposure group（ 10.0 mg/kg）, low-dose arsenic exposure group （0.4 mg/kg）, and control group. The rats were exposed by drinking arsenic solution which was mixed with distilled water. Rats were weighed every 10 days and dose volume of arsenic solution was adjusted. After continuous exposure for 4 months, blood and urinary arsenic were determined. Rat kidneys were taken and stained by Immunohistochemistry SABC. PKC-a positive cells in the kidney were observed and counted, and its average gray value was analyzed with image analysis software（Biomias）. Results Proximal tubules PKC-α-positive cell count [ （3.62 ＋ 1.90）, （10.07 ± 3.22）/field], glomerular PKC-α-positive cell count[ （3.62± 1.90）, （10.07 ± 3.22）/field] in high and low arsenic group ofSD rat kidney were lower than those of the control group[（60.00 ± 9.63）, （18.57 ± 2.71/field, all P 〈 0.05]; both urinary arsenic level[ （7366.62 ± 1086.50）, （1744.31 ± 300.12）μg/L] and blood arsenic level[（31.59 ± 9.24）, （16.58± 2.08）lxg/L] in high-dose and low-dose groups were higher than those of the control group（18.97 ± 3.58）, （18.97 ± 3.58）lxg/L, all P 〈 0.05]; the average gray values of SD rat kidney proximal tubule, glomerular PKC-ot positive cells in high-dose and low-dose groups（ 142.79 ± 11.16, 122.15 ±5.91 ） were higher than that of the control group （114.33 ±6.70, all P 〈 0.05）. Conclusions Arsenic can decrease SD rat kidney PKC-ot -positive cells. The regulatory function of PKC-α in inhibiting cell apoptosis of.kidney of rats with arsenic poisoning is weakened.