羟基红花黄色素A、红花提取物及复方脑得生片中羟基红花黄色素A在大鼠体内的排泄比较研究

Comparison of Excretion of Hydroxysafflor Yellow A after Oral Administration of Monomer,Medicinal Substance Aqueous Extract and Naodesheng Tablets in Rats

目的研究比较大鼠口服羟基红花黄色素A单体、红花提取物及复方脑得生片后羟基红花黄色素A胆汁及尿、粪排泄动力学差异。方法采用反相高效液相色谱法测定给药后大鼠胆汁及尿、粪中羟基红花黄色素A的含量,分别计算羟基红花黄色素A的累计排泄量及排泄率。结果灌胃给予羟基红花黄色素A单体、红花提取物和复方脑得生片后24 h的胆汁累计排泄率分别为(0.164±0.072)%、(0.125±0.044)%、(0.056±0.016)%;尿中累计排泄率分别为(0.075±0.004)%、(0.156±0.01)%、(0.024±0.002)%;粪中累计排泄率分别为(46.7±20.4)%、(54.5±18.8)%、(26.8±8.61)%,其累计排泄率在各组间均有显著性统计学意义。结论羟基红花黄色素A单体、红花提取物、复方脑得生片中羟基红花黄色素A在大鼠体内的排泄存在明显差异,显示红花中的其他成分影响了羟基红花黄色素A在体内的吸收与利用,而复方配伍后可增加红花中羟基红花黄色素A在体内的利用程度,减少羟基红花黄色素A以原型排出体外。

OBJECTIVE To compare the excretion of hydroxysafflor yellow A after oral administration of hydroxysafflor yellow A, Safflower extract and Naodesheng tablets in rat. METHODS Bile, urine and feces were collected within 24 h after oral adminstration of hydroxysafflor yellow A, Safflower extract and Naodesheng tablets repectively, and then determined by RP-HPLC. RESULTS Cu- mulative biliary excretion ratios within 24 h at a dose of 50 mg kg- 1 after intragastrie administration of monomer, medicinal substance extract and Naodesheng accounted for （0. 164 ± 0. 072）%, （0. 125 ± 0. 044 ）% and （0. 056 ± 0. 016 ）% of the dose respectively; Cu- mulative urinary excretion ratios accounted for （ 0. 075 ± 0. 004 ） % , （ 0. 156 ± 0. 01 ） % and （ 0. 024 ＋ 0. 002 ） % , respectively ; Cumu- lative feces excretion ratios accounted for （46. 7 ± 20. 4 ） % , （ 54. 5 ± 18.8 ） % and （ 26. 8 ± 8.61 ） % , respectively. Cumulative ex- cretion ratios by the three routes showed significant differences between reference group and the other groups. CONCLUSION The results indicated that the other ingredients of Safflower had remarkable influence on the absorption and utilization of hydroxysafflor yellow A. The compatibility promotes the absorption and ntilization of hydroxysafflor yellow A, reduces the excretion of hydroxysafflor yellow A in form of proto-drug.