冷库中低温细菌的分离及鉴定

Isolation and Identification of Cold—adapted Bacteria in Cold Storage

从来自冷库中的样品中共筛选分离出5株低温细菌。对分离的低温细菌进行了分类鉴定,主要进行了生理、生化特性分析,对其中的2株细菌作了16S rDNA测序并通过BLAST比对进行了鉴定。生化鉴定结果表明:所有分离到的低温细菌均为革兰氏阴性菌。经16S rDNA鉴定的3株低温细菌全部为假单孢菌属的菌株。

This paper firstly selects 5 cold-adapted microorganisms from the samples in cold storage,and then identifies them according to their morphological and physiological characteristics,two of which 16S rDNA-sequencing are made and identified by means of BLAST matching.The biochemical identification results show that all the isolated low-temperature bacteria are gram-negative bacteria;3 col-adapted bacteria identified by 16S rDNA are stains of Pseudomonas spp.