CTLA-4基因多态性与豫南地区汉族人群1型糖尿病的相关性

Association between CTLA-4 gene polymorphism and type 1 diabetes mellitus in Han population in the south of Henan province

目的探讨细胞毒性T淋巴细胞相关抗原4(CTLA-4)基因第一外显子+49 A/G的多态性与豫南地区汉族人群1型糖尿病(T1DM)的相关性。方法采用PCR-RFLP技术分析108 T1DM患者及100例正常对照组的CTLA-4基因第一外显子+49 A/G多态性。结果 T1DM组等位基因+49G频率明显高于正常对照组(P=0.001),基因型GG明显高于正常对照组(P=0.005)。结论 CTLA-4基因第一外显子+49 A/G多态性与豫南地区T1DM易感性相关。

Objective To investigate the association of the first exon of cytotoxic T lymphocyte-associated antigen 4（CTLA-4） gene ＋49A/G polymorphism with susceptibility to type 1diabetes mellitus（T1DM） in Han population in the south of Henan province.Methods The A / G phenotype at position 49 of the first exon of CTLA-4 gene was determined by polymerase chain reaction restriction fragment length polymorphism（PCR-RFLP） method in 108 T1DM patients and 100 healthy control subjects.Results The frequencies of the ＋ 49G allele and GG genotype were significantly higher in T1DM patients than those in control subjects（P = 0.001 vs 0.005,respectively）.Conclusion The polymorphism of the first exon of CTLA-4 gene ＋ 49A / G confers susceptibility to T1DM in the south of Henan province.