细胞黏附分子CD44S、CD44V5在口腔鳞癌中的表达及意义

The expression and significance of cellular adhesive molecular CD44S and CD44V5 in oral squamous cell carcinoma

目的探讨口腔鳞癌(OSCC)中细胞黏附分子CD44S、CD44V5的表达及其临床意义。方法应用免疫组化SP法检测12例(新鲜标本)正常口腔组织(NOT)及59例(20例为新鲜标本)手术切除的OSCC中细胞黏附分子CD44S及CD44V5的表达。RT-PCR法检测12例NOT及20例OSCC中CD44SmRNA的表达。结果 CD44S、CD44V5和CD44SmRNA在OSCC中的阳性表达率明显高于其在NOT中的表达,且差异有统计学意义(P<0.05)。CD44S和CD44V5的表达与肿瘤的病理分级、临床分期及淋巴结转移有关,而在性别、年龄及肿瘤大小方面的差异无统计学意义。结论 CD44S和CD44V5的异常表达与OSCC的病理分级、临床分期及淋巴结转移密切相关,其可作为预测OSCC侵袭和转移潜能的指标。

Objective To explore the expression and clinical significance of cellular adhesive molecular CD44S and CD44V5 in oral squamous cell carcinoma（OSCC）.Methods The expression of CD44S and CD44V5 were examined by immunohistochemical SP method in 12 cases of normal oral tissue（NOT） and 59 cases of OSCC.The expression of CD44S mRNA in 20 cases of OSCC and 12 cases of NOT were detected by RT-PCR.Results The positive expression rates of CD44S,CD44V5 and CD44SmRNA in OSCC were significantly higher than those in NOT.There was significant difference between the two groups（P0.05）.The expression of CD44S and CD44V5 were correlated with pathological grade,clinical stage,lymph node metastasis,but it was not associated with sex,age and tumor size.Conclusion The expression of CD44S and CD44V5 are closely related with pathological grade,clinical stage and lymph node metastasis of OSCC.It can be used as marker to predict potential of metastasis and invasion of OSCC.