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OsBTF3转基因水稻对病原菌侵染的反应和防卫基因的表达分析 被引量：5

Responses and expression of defense genes of transgenic rice with over-expressed or RNAi-silenced OsBTF3 gene upon bacterial infection
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摘要 为了阐明OsBTF3基因在水稻对白叶枯病菌(Xanthomonas oryzae pv.oryzae,Xoo)和细菌性条斑病菌(X.oryzae pv.oryzicola,Xoc)侵染抗/感反应中的功能,本研究以OsBTF3过量表达和RNAi转基因株系为材料,比较分析了T1代株系对病菌侵染的抗感病反应,定量测定了防卫基因的表达动态。结果表明,与野生型对照株相比,无论过量表达株系,还是RNAi株系对Xoo和Xoc侵染均表现为更加感病的反应;经Xoo接种后,过量表达和RNAi株系OE37和RNAi株系RI30防卫基因的表达发生了不同程度的变化。因此,OsBTF3增量/减量表达对水稻抗/感病性具有重要的调控作用,这种作用可能并不依赖于水杨酸(SA)和茉莉酸(JA)介导的信号途径。 To reveal the functions of OsBTF3 gene in rice in response to infection by Xanthomonas oryzae pvs. oryzae and oryzicola, the responses and expression of defense genes of transgenic rice with over-expressed or RNAi- silenced OsBTF3 were comparably analyzed. The results showed that more susceptible phenotypes were found either in transgenic rice with over-expressed or RNAi-silenced OsBTF3 than the control of wildtype plants. In addition, the changes in expression of selected defense genes induced by Xoo were observed both in the transgenic rice lines and the control plants. Therefore, over- or down-expression of OsBTF3 regulates the disease resistance/susceptibili- ty in rice, which is independent on SA- or JA-mediated signaling pathways.
作者 李广旭 陈华民 吴茂森 何晨阳
机构地区 中国农业科学院植物保护研究所 辽宁省农业科学院果树研究所
出处 《植物病理学报》 CAS CSCD 北大核心 2012年第2期169-175,共7页 Acta Phytopathologica Sinica
基金 国家转基因生物新品种培育重大专项(2009ZX08009-044B 2011ZX08001-002)
关键词 水稻 OsBTF3 转基因 感病性 防卫基因表达 rice OsBTF3 transgenic disease susceptibility defense gene expression
分类号 S432.1 [农业科学—植物病理学]
  • 相关文献

参考文献27

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二级参考文献14

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共引文献8

同被引文献87

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引证文献5

二级引证文献9

植物病理学报
2012年 第2期

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