陇油6号油菜BnMPK6基因启动子分离及序列分析

Isolation and sequence analysis of BnMPK6 gene promoter from brassica napus(rape) longyou 6

根据已知的陇油6号油菜BnMPK6基因序列设计三个特异引物SP1,SP2,SP3,通过染色体步移技术,获得了陇油6号油菜BnMPK6基因起始密码子ATG上游启动子序列1 337bp.用PlantCARE在线分析等软件分析此序列,发现该序列具有启动子的基本元件CAAT-box和7个抗寒、ABA、胁迫响应元件CANNTG,1个抗寒、抗旱、ABA响应元件CCGAC,2个缺氧性功能中最基本的顺势调控元件ARE,2个赤霉素敏感元件GAREmotif,1个低温应答顺势调控元件LTR及2个干旱诱导的mby结合部位MBS.序列分析表明,该启动子序列是一个强的逆境胁迫诱导启动子,特别是一个强的寒冷、干旱胁迫诱导启动子.

In this study,BnMPK6 gene promoter which from Brassica napus（rape） longyou 6 is isolated by chromosomal walking.1 337 bp sequence is obtained by sequencing.The functional elements are analysed by PlantCARE.Bioinformatical analysis of this sequence shows that the promoter contains some typical elements：CAAT-box,stress-induced elements,chill-proof and ABA stress responsed element,chill-proof,ABA and drought control respondsed element,cis-acting anaerobic regulatory element,gibberellin-responsive element and cis-acting low-temperature respondsed element.Furthermore,there is a mby binding site involved in drought-inducibility MBS in the promoter.Sequence analysis indicates that the promoter is a strong adversity stress-induced promoter,especially in cold and drought.