重组人血管内皮抑制素对多发性骨髓瘤内皮细胞增殖和迁移的影响

Effects of recombinant human endostatin on proliferation and migratory of vascular endothelial cells in mutiply myeloma

目的观察重组人血管内皮抑制素注射液(内皮抑素)对人脐静脉内皮细胞株(HUVEC)和多发性骨髓瘤细胞株KM3抑制增殖及诱导凋亡的作用,研究其对KM3细胞培养上清诱导的HUVEC迁移效果的影响。方法 WST-1法观察不同浓度的内皮抑素分别对HUVEC和KM3细胞株的增殖抑制作用,Annexin V-FITC法检测有效浓度的内皮抑素对HUVEC和KM3细胞株的凋亡作用,改良的Boyden小室法检测内皮抑素对KM3细胞株培养上清诱导的HUVEC迁移的影响。结果 50～500 ng/mL内皮抑素对HUVEC具有抑制增殖作用,而对KM3细胞未见明显作用。100、200 ng/mL的内皮抑素对HUVEC具有诱导凋亡作用;而对KM3细胞未见明显作用。与不加药组相比,内皮抑素能抑制KM3细胞培养上清促内皮细胞迁移的作用(P<0.01)。结论内皮抑素能抑制HUVEC的增殖及诱导其凋亡,并能抑制骨髓瘤细胞的促内皮细胞迁移作用。

To observe the effect of inhibiting proliferation and inducing apoptosis effect of recombinant hu- man endostatin （ES） on the human umbilical vein endothelial cells （HUVECs） and myeloma cell lines KM3, and investigate its influence on HUVEC immigration induced by KM3 cell cultured supematant. Methods Cell proliferation in different concentration of ES was determined by WST-1 assay. The fluorescence flow cytometry （ FCM ） Annexin VFITC assay was applied to detect the changes of apoptotic cells. Modified Boyden chamber assay was used to observe the migration activity of HUVEC induced by KM3 cells. Results 50-500 ng/mL ES inhibited HUVEC proliferation, but had no influence on KM3 cell lines. 100, 200 ng/mL ES induced HUVEC apoptosis, but had no effect on KM3 cell lines. ES inhibited the endothelial cell migration induced by KM3 cell cultrued supematant（P 〈 0.01 ）. Conclusion ES can not only inhibit the proliferation and induce apoptosis of HUVEC cells but also inhibit endothelial cell migration ability of multiple myeloma cells.