健脾解毒方逆转大肠癌多药耐药基因的研究

multidrug resistance gene of cancer of colon reversed by Jianpijiedu Herb

目的从分子生物学的角度探讨健脾解毒方逆转大肠癌多药耐药基因的作用机制,为中医健脾解毒法治疗大肠癌提供理论依据。方法应用健脾解毒方药对SD大鼠进行灌胃,采集动脉血制备健脾解毒方药物血清,在体外培养人结肠直肠腺癌耐长春新碱细胞HCT-8/V,分别加入培养液配制的5%、10%、15%、20%健脾解毒方药物血清,采用MTT方法检测各组HCT-8/V细胞生长抑制率。结果健脾解毒方药物血清对HCT-8/V细胞有抑制作用,随浓度升高、时间延长,其抑制作用增强,呈剂量和时间效应关系。20%的药物血清和VCR在24、48、72 h对肿瘤细胞的生长抑制率均有统计学差异(P<0.01),随作用时间延长,对肿瘤细胞的杀伤作用明显增强。结论健脾解毒方可逆转大肠癌多药耐药基因,并伴随相关基因的调控。

To investigate the mechanism of Jianpijiedu herb reversing muhidrug resistance gene of cancer of colon, and to provide a theoretical basis for treatment of colorectal cancer. Methods The drug serum of Jianpijiedu Herb was prepared with SD rats. HCT-8/V cells were cultured in vitro. HCT-8/V cells growth inhibition with 5% , 10%, 15% and 20% of the drug serum was measured by MTI＂ method. Results Jianpijiedu herb inhibited HCT-8/V cell growth in a timeand dosedependent manner. The inhibition of cell growth rates were significantly different with 20% of the drug serum of Jianpijiedu Herb and VCR by 24 h, 48 h and 72 h （ P 〈 0.01 ）. The killing effect on tumor cells was significantly enhanced with prolongeel time. Conclusion Jianpijiedu herb might reverse multidrug resistance gene of cancer of colon in the control of related gene.