血站利用O型血液中去除的淋巴细胞制备细胞因子诱导的杀伤细胞的可行性

The feasibility to prepare cytokine-induced killer cells using the lymphocytes removed from 0 type blood inthe blood bank

目的探讨血站利用0型血液中去除的淋巴细胞制备细胞因子诱导的杀伤细胞（CIK）的可行性。方法采集30例0型献血者全血，利用白膜法4—6h内提取白细胞，然后用密度梯度离心法分离出淋巴细胞，再经分化、诱导、培养从而制备CIK细胞。结果30例0型献血者血液中去除的淋巴细胞制备CIK含量为（1．304-0．12）X1010／L，且cD3＋／CD56＋细胞表达占40％一60％，冰冻保存1个月后回收率达90％以上。结论利用0型无偿献血者血液中去除的淋巴细胞制备CIK，既利于保证原料血液的质量，又利于保证CIK细胞制品的质量和数量，可最大限度降低CIK的制备成本。

Objective To investigate the feasibility of preparing cytokine-induced killer cells （CIK） using the lymphocytes removed from O type blood in the blood bank. Methods The whole blood was collected from 30 blood donors with 0 type blood. The white blood cells were enriched and were seperated by density-grediant centrifugation. The lymphocytes were differentiated, induced and cultured to prepare CIKs. Results The CIKs produced from the 30 cases of blood donors with type O blood was （ 1.30 _＋ 0. 12） x 1010＋/L with CD3 ＋/CD56 ＋ population up to 40% - 60%. The recovery rate was up to over 90% after 1 month of cryopreservation. Condusion Preparing the CIK with the lymphocytes removed from the type 0 blood donation is an ecnomical way to cut the cost of CIK preparation.