液相色谱-串联质谱法测定猪肉中3种β-受体激动剂类药物残留

Determination of 3 β-agonists in Pork by Liquid Chromatography-tandem Mass Spectrometriy

试验建立了液相色谱-串联质谱(LC-MS/MS)检测猪肉中的克仑特罗、沙丁胺醇、莱克多巴胺残留的方法,并对方法进行了优化。样品经0.2mol/L的乙酸铵缓冲液提取,加入β-葡萄糖醛苷酶进行酶解,提取液经PCX柱进行净化,液相色谱-串联质谱进行测定,内标法定量。结果显示,猪肉中3种β-受体激动剂残留浓度在0.2～10μg/kg范围线性关系良好,线性相关系数分别为0.9997、0.9999和0.9993,定量检出限均为0.5μg/kg,回收率分别为93.57%～96.63%、92.35%～96.01%和91.66%～96.54%,该方法灵敏度高、重现性强,适用于猪肉组织样品中克仑特罗、沙丁胺醇及莱克多巴胺的确证检测。

To established a method to detect the residues of clenbuterol,salbutamol and ractoPamine in pork by liquid chromatography-tandem mass spectrometry （LC-MS/MS） and optimized it. The samples was treated by 0.2 mol/L ammonium acetate buffer and added the beta-glucuronidase to it, purified the extracting solution with PCX column, determined by LC-MS/MS and quantitated with internal standard method. The results showed that the residual concentration of the three kinds of β-agonists in pork was 0.2 to 10.0μg/kg and the linear correlation was well. The linear correlation coefficient were 0. 9997,0. 9999 and 0. 9993,respectively. We detected the limitative concentration was 0. 5 μg/kg and the coefficient of recovery were 93.57 to 96.63 %, 92.35 % to 96.01% and 91.66 % to 96.54 %, respectively. This method has the advantages of good sensitive, accuracy and easy repetition and it is suitable for the detection of clenbuterol, salbutamol and ractopamine in pork.