摘要
目的观察FGF10及其受体FGFR2Ⅲb在小鼠肾脏发生发育中的表达和分布,探讨其在肾脏发生发育中的作用。方法选取胚龄12、14、16及18 d和出生后1、7、14、21及42 d小鼠肾组织,用免疫组织化学和Western blot,检测FGF10和FGFR2Ⅲb的表达。结果胚龄12 d组FGF10及FGFR2Ⅲb开始在输尿管芽微弱表达;随着肾脏发育成熟,FGF10及FGFR2Ⅲb主要表达于远端小管和集合管;FGF10在近曲小管表达,近直小管无表达;FGFR 2Ⅲb在近端小管无阳性表达;生后肾组织及发育各阶段肾小体未见FGF10及FGFR2Ⅲb表达。Western blot显示随着胚(日)龄的增加,FGF10及FGFR2Ⅲb在肾脏的表达量先增后减。结论 FGF10和FGFR2Ⅲb在肾脏发生发育过程中发挥重要作用。
Objective To observe the expression and location of FGF10 and FGFR2 Ⅲb during kidney development of mouse in order to investigate their roles in kidney development.Methods Immunohistochemistry technique and Western blotting method were used to examine the expression of FGF10 and FGFR2 Ⅲb at embryonic day 12、14、16、18 and neonatal day 1、7、14、21、42 in the developing mouse kidney.Results FGF10 and FGFR2 IIIb were weakly expressed in the ureteric buds at E12d and then with the renal development,FGF10 and FGFR2 IIIb were expressed mainly in distal tubules and collecting ducts.FGF10 was expressed also in the proximal convoluted tubule.FGFR2 IIIb was not expressed in the proximal tubule.The renal corpuscles in each development stage was not positive for expression of FGF10 and FGFR2 IIIb.Western blot showed that during kidney development,the expressions of FGF10 and FGFR2 IIIb were increased firstly and then decreased.Conclusions FGF10 and FGFR2 IIIb probably play an important role in the renal development.
出处
《基础医学与临床》
CSCD
北大核心
2012年第4期437-441,共5页
Basic and Clinical Medicine
基金
辽宁省教育厅科研项目(L2010274)
