VIPR-1基因5′调控区多态位点与鸡就巢性的关联分析

Association of Polymorphisms in the 5′ Regulator Region of the VIPR-1 Gene with Chicken Broodiness

本研究旨在探索血管活性肠肽I型受体(Vasoacitve intestinal peptide type I receptor,VIPR-1)基因5′调控区(-496～-1bp)多态性及其单倍型效应对鸡就巢性的影响,寻找影响肉鸡就巢性的分子标记,为降低或者剔除肉鸡就巢行为的育种研究提供相应依据。采用测序技术对498只清远麻鸡VIPR-1基因5′调控区进行多态性检测及基因型分析,利用最小二乘均数对VIPR-1基因5′调控区的多态位点与就巢性状进行相关分析,利用PHASE软件对多态位点进行单倍型分析。结果,VIPR-1基因5′调控区存在12个多态性位点,G-359T、G-266T、A-134G、A-94G、C-72G对18～43周龄的就巢持续天数影响达到显著水平(P<0.05),A-94G位点对18～54周龄的就巢率的影响达到显著水平(P<0.05);构建的单倍型对18～54周龄的就巢持续天数的影响达到极显著水平(P<0.01)。最小二乘分析结果表明,CCTGGGAAGCAG/TTGGGGAAGCAC型个体的就巢持续天数比其它单倍型个体极显著的延长(P<0.01)。结果表明,VIPR-1基因5′调控区(-496～-1bp)可能存在影响鸡就巢行为的分子标记,CCTGGGAAGCAG/TTGGGGAAGCAC型个体对鸡就巢性具有较大的遗传效应,可作为分子标记应用于家鸡就巢行为的筛选。

Abstract. The objective of this study was to investigate the effects of polymorphisms or haplo- types in the 5r regulatory region （--496-1 bp） of the vasoactive intestinal peptide type I recep- tor （VIPR-1） gene on broodiness in Qingyuan Partridge chicken, and then to look for the genetic marker inhibiting broodiness. The polymorphisms in the 5＇regulatory region of the VIPR-1 gene were detected by directly sequencing in 498 Qingyuan Partridge chicken under cage condition. The association between mutation sites and broodiness was analyzed by the least square mean analysis. The PHASE software was used for haplotype association analysis. A total of 12 SNPs were found in --496-1 bp region of the VIPR-1 gene. Significant associations （P〈0.05） were found between G-359T, G-266T, A-134G, A-94G, C-72G and duration of broodiness from 18- to43-week-old. A-94G was significantly associated with broody frequency （%） from 18- to 54- week-old （P〈0.05）. Haplotypes based on 12 polymorphism sites in --496-1 bp of VIPR-1 gene significantly associated with duration of broodiness from 18- to 54-week-old （P〈0.01）. The least square analysis showed that the birds with CCTGGGAAGCAG/TTGGGGAAGCAC had significantly longer duration of broodiness than those with other haplotypes （P〈0.01）. Marker- trait association analysis indicated that genetic marker for chicken broodiness in the 5＇ regulatory region of VIPR-1 gene may be exist. The individuals with CCTGGGAAGCAG/TTGGGGAAG- CAC had a great genetic effect on broodiness in Qingyuan Partridge chicken, which can be a can- didate marker for molecular marker-assisted selection.