小鼠出生后背部毛囊发育与Lef-1表达的研究

The Development of Dorsal Hair Follicle and Expression of Lef-1 in Postnatal Murine

旨在研究Lef-1在小鼠背部毛囊生长周期中的表达规律及其与毛囊生长更替的关系。本试验采用石蜡切片HE染色、Western blotting以及免疫组织化学方法,研究小鼠毛囊的周期性变化,检测Lef-1在小鼠毛囊生长的不同时期皮肤组织中的蛋白表达水平,并对其进行组织定位。结果,昆明系小鼠毛囊生长呈周期性变化,处于不同生长时期的毛囊具有各期的特殊结构;Western blotting结果显示,在各阶段皮肤组织中存在Lef-1蛋白的表达,其在毛囊生长初期的表达量显著高于中期和末期;免疫组织化学结果显示,Lef-1在不同毛囊生长时期的皮脂腺、根鞘和真皮乳头均有不同程度的表达。Lef-1的表达与毛囊的周期性变化存在相关性。

To study the histological characteristics of murine hair follicle and the function of Lef-1 at different hair follicle growth stages, the expression and localization of Lef-1 were investigated. HE staining was used to observe the morphology of the murine hair follicle at each stage, West- ern blotting and immunohistochemistry were used to determine the expression level and localiza- tion of Lef-1 protein at different hair follicle cycle stages. The result suggested that the gowth of murine hair follicle showed cyclic variation. For each stage, murine hair follicle had stage specific characteristics. The result of Western blotting showed that Lef-1 was expressed in murine skin at different growth stages, and the expression level in hair follicle early growth period was higher than that in hair follicle middle and late growth periods. The result of immunohistochemistry showed that Lef-1 was expressed in sebaceous gland, root sheath and dermal papilla of hair folli- cle at different growth stages in different degree. The results indicate that cyclic variation of hair follicle depend on the expression level of Lef-1.